# Center for Macromolecular Interactions ![CMI instruments and logo](/sites/g/files/omnuum4016/files/2025-06/cmi_header_2025new_extra_narrow_0.png) ## The Center for Macromolecular Interactions The Center for Macromolecular Interactions (CMI) in the department of [Biological Chemistry and Molecular Pharmacology](http://bcmp.med.harvard.edu/) at [Harvard Medical School](http://hms.harvard.edu/) has a mission to enhance basic research in the HMS community by providing scientific consultation, training and access to [shared biophysical instruments](/technologies "Technologies") for the characterization and analysis of macromolecules and their complexes. CMI resources include tools for characterizing [molecular properties](/measuring-molecular-properties "Measuring Molecular Properties"), for measuring [molecular interactions](/measuring-molecular-interactions "Measuring Molecular Interactions") and for [protein production](/protein-production "Protein Production"). The facility currently offers training and access to instruments for [Isothermal Titration Calorimetry (ITC)](/isothermal-titration-calorimetry "Isothermal Titration Calorimetry (ITC)"), [Surface Plasmon Resonance (SPR)](/surface-plasmon-resonance "Surface Plasmon Resonance (SPR)"), [Biolayer Interferometry (BLI)](/biolayer-interferometry "Biolayer Interferometry (BLI)"), [MicroScale Thermophoresis (MST)](/microscale-thermophoresis "MicroScale Thermophoresis (MST)"), [Differential Scanning Fluorimetry (DSF)](/differential-scanning-fluorimetry "Differential Scanning Fluorimetry (DSF)"), [Circular Dichroism (CD)](/circular-dichroism "Circular Dichroism (CD)"), [Light Scattering](/light-scattering "Light Scattering"), including [size-exclusion chromatography with multi-angle light scattering (SEC-MALS)](/sec-mals "Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)"), [Dynamic Light Scattering (DLS)](/dynamic-light-scattering "Dynamic Light Scattering (DLS)"), [Mass Photometry (MP)](/mass-photometry "Mass Photometry (MP)"), and protein production, including [Fast Protein Liquid Chromatography (FPLC)](/fast-protein-liquid-chromatography "Fast Protein Liquid Chromatography (FPLC)") and [Cell-free Protein Synthesis](/cell-free-protein-synthesis "Cell-Free Protein Synthesis"). The CMI is primarily a shared instrument facility offering [training and access](/training "Training") on our [technologies](/technologies "Technologies"). The CMI also offers *limited* [data collection services](/services "Services"), including protein chromatography, select characterization of protein physical properties and yeast surface display [nanobody selections](/nanobody-services "Nanobody Services"). ## CMI News [### CMI Rates for FY27 Posted ](/news/2026/06/cmi-rates-fy27-posted) June 09, 2026 New CMI fees for access, training and services, effective July 1, 2026, have been posted. [### Tecan Spark Plate Reader Training and Access now available ](/news/2026/04/tecan-spark-plate-reader-training-and-access-now-available) April 16, 2026 The CMI Tecan Spark Multimode Plate Reader is available for training and access. The plate reader is configured with Absorbance mode, Luminescence mode, and enhanced Fusion Optics Fluorescence mode, and supports a wide array of assays in microplate format... [### FPLC Training and Access Now available ](/news/2025/10/fplc-training-and-access-now-available) October 22, 2025 The CMI AKTA Pure is available for training and access. Select columns for size-exclusion and ion exchange are also available for shared use: Superdex 200 10/300, Superdex 75 10/300, Superose 6 10/300, Mono Q 5/50, Mono S 5/50. [ More CMI News arrow\_circle\_right ](/news) ## CMI Events [### Demo of Refeyn MassFluidix HC device ](/event/demo-refeyn-massfluidix-hc-device?occ_id=0)Jul. 16 - Jul 17, 2026 | All day The CMI is hosting a demo of the Refeyn MassFluidix HC device. CMI Users who are interested in participating in the demo July 16-17, should fill out this Refeyn MassFluidix HC Demo form. About Refeyn MassFluidix HC: Refeyn’s microfluidics system... [### Demo of Sartorius Octet R8e ](/event/demo-sartorius-octet-r8e?occ_id=0)Jul. 27 - Jul 29, 2026 | All day Stay tuned for more information about our demo of the Sartorius Octet R8e. ## Newest Technologies at the CMI [### Multimode Plate Reader ](/multimode-plate-reader)Spark Multimode Plate Reader from Tecan, configured with Absorbance mode, Luminescence mode, and enhanced Fusion Optics Fluorescence mode. ![tecan spark](/sites/g/files/omnuum4016/files/styles/hwp_16_9__480x270/public/2026-01/16x9_tecanspark_crop.png?itok=SfIyuPVT) [### Cell-Free Protein Synthesis ](/cell-free-protein-synthesis)The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip. ![Nuclera eProtein Discovery](/sites/g/files/omnuum4016/files/styles/hwp_16_9__480x270/public/2026-01/16x9_nuclera_crop.png?itok=AhpNwBgK) [### Fast Protein Liquid Chromatography (FPLC) ](/fast-protein-liquid-chromatography)The AKTA Pure from Cytiva is a preparative liquid chromatography system, used for protein purification by Size-Exclusion Chromatography and Ion Exchange. ![AKTA Pure](/sites/g/files/omnuum4016/files/styles/hwp_16_9__480x270/public/2025-05/16x9%20AKTA%20Pure.png?itok=vr5b9QcY) [### Mass Photometry (MP) ](/mass-photometry)The TwoMP from Refeyn for Mass Photometry is a single-particle static light scattering detector, used to measure molar mass in solution. ![Refeyn TwoMP](/sites/g/files/omnuum4016/files/styles/hwp_scale_small/public/2025-02/Refeyn%20TwoMP_crop.png?itok=EWEKMl1U) arrow\_back arrow\_forward ## Recent CMI User Publications - CMI users agree to [acknowledge](/resource/acknowledgments "Acknowledgments") the support of **"the Center for Macromolecular Interactions at Harvard Medical School*****"*** in publications arising from use of the facility. **CMI RRID: SCR\_018270.** Download 252 citations download- [BibTeX](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=tagged) - [Marc](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=marc) - [PubMedId](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=standard_pager&number_of_items=3&sort_field=bibcite_year--desc&taxonomy_filters%5Bfield_hwp_c_binding%5D&&&format=ris) Sirimanakul, Suwitchaya, Joseph D. Hurley, Nakarin Vutipow, Fabien Loison, Anongnard Kasorn, Lueacha Tabtimmai, Pinpunya Riangrungroj, Jeerapond Leelawattanachai, Teetouch Ananwattanasuk, Napachanok Swainson, Kiattawee Choowongkomon, Andrew C. Kruse, and Dujduan Waraho-Zhmayev. [2026] 2026. “[A Simple and Cost-Effective Electrochemical Assay for Heart Failure Prognosis Using Soluble Suppression of Tumorigenicity 2-Specific Nanobodies](/publication/simple-and-cost-effective-electrochemical-assay-heart-failure-prognosis-using-soluble)”. *ACS Pharmacology & Translational Science*. doi:10.1021/acsptsci.6c00082. Sirimanakul, Suwitchaya, Joseph D. Hurley, Nakarin Vutipow, Fabien Loison, Anongnard Kasorn, Lueacha Tabtimmai, Pinpunya Riangrungroj, Jeerapond Leelawattanachai, Teetouch Ananwattanasuk, Napachanok Swainson, Kiattawee Choowongkomon, Andrew C. Kruse, and Dujduan Waraho-Zhmayev. [2026] 2026. “[A Simple and Cost-Effective Electrochemical Assay for Heart Failure Prognosis Using Soluble Suppression of Tumorigenicity 2-Specific Nanobodies](/publication/simple-and-cost-effective-electrochemical-assay-heart-failure-prognosis-using-soluble)”. *ACS Pharmacology & Translational Science*. doi:10.1021/acsptsci.6c00082. - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://doi.org/10.1021/acsptsci.6c00082) Heart failure (HF) is a major global health concern, particularly in low- and middle-income countries where it is often underdiagnosed. Prognostic testing plays a crucial role in guiding treatment and monitoring disease progression. In this study, we... - [ descriptionPublisher's Version](https://doi.org/10.1021/acsptsci.6c00082) de Puig, Helena, Erkin Kuru, Michaël Moret, Allison Flores, Suneesh Karunakaran, Dinara Sayfullina, Subhrajit Rout, Carmen Escobedo-Lucea, James J. Collins, and George M. Church. [2026] 2026. “[Genetic Code Expansion Enables Programmable Covalent Protein Design](/publication/genetic-code-expansion-enables-programmable-covalent-protein-design)”. *BioRxiv* (2026.05.15.725538). doi:10.64898/2026.05.15.725538. de Puig, Helena, Erkin Kuru, Michaël Moret, Allison Flores, Suneesh Karunakaran, Dinara Sayfullina, Subhrajit Rout, Carmen Escobedo-Lucea, James J. Collins, and George M. Church. [2026] 2026. “[Genetic Code Expansion Enables Programmable Covalent Protein Design](/publication/genetic-code-expansion-enables-programmable-covalent-protein-design)”. *BioRxiv* (2026.05.15.725538). doi:10.64898/2026.05.15.725538. - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2026/05/16/2026.05.15.725538.full.pdf) Covalent chemistry has transformed small-molecule drug discovery, yet analogous strategies for proteins remain largely inaccessible because covalent warheads cannot be readily integrated into biologics. Conventional genetic code expansion requires... - [ descriptionPublisher's Version](https://www.biorxiv.org/content/biorxiv/early/2026/05/16/2026.05.15.725538.full.pdf) Ragucci, Adelyn E, Sadie P Antine, Ethan M Leviss, Sarah E Mooney, Jasmine M Garcia, Lena Shyrokova, Vasili Hauryliuk, Amy S Y Lee, and Philip J Kranzusch. [2026] 2026. “[Nuclease-NTPase Antiphage Defence Systems Use Conserved Molecular Features to Control Bacterial Immunity.](/publication/nuclease-ntpase-antiphage-defence-systems-use-conserved-molecular-features-control)”. *Nature Microbiology*. doi:10.1038/s41564-026-02312-8. Ragucci, Adelyn E, Sadie P Antine, Ethan M Leviss, Sarah E Mooney, Jasmine M Garcia, Lena Shyrokova, Vasili Hauryliuk, Amy S Y Lee, and Philip J Kranzusch. [2026] 2026. “[Nuclease-NTPase Antiphage Defence Systems Use Conserved Molecular Features to Control Bacterial Immunity.](/publication/nuclease-ntpase-antiphage-defence-systems-use-conserved-molecular-features-control)”. *Nature Microbiology*. doi:10.1038/s41564-026-02312-8. - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.doi.org/10.1038/s41564-026-02312-8) Bacteria encode diverse defence systems, including restriction-modification and CRISPR-Cas, that cleave nucleic acid to protect against phage infection. Bioinformatic analyses demonstrate that many recently identified antiphage defence operons comprise a... - [ descriptionPublisher's Version](https://www.doi.org/10.1038/s41564-026-02312-8) - Previous page chevron\_left - [1](?page=0 "Current page") - [2](?page=1 "Go to page 2") - [3](?page=2 "Go to page 3") - [4](?page=3 "Go to page 4") - [5](?page=4 "Go to page 5") - [6](?page=5 "Go to page 6") - [7](?page=6 "Go to page 7") - [8](?page=7 "Go to page 8") - [9](?page=8 "Go to page 9") - [ Last page 84 ](?page=83 "Go to last page") - [ Next page chevron\_right ](?page=1 "Go to next page") [ CMI User Publications arrow\_circle\_right ](/publications)