# Data Management ## expand\_more ### About CMI Data Files #### Users are responsible for storage of all raw and processed data collected at the CMI. - Users should have a plan to copy or transfer all raw and process data to their own local or cloud storage system. - While the CMI allows temporary local storage of CMI User data on the instrument computer, **we make no guarantees on the security or long-term availability of any data at the CMI**. - For most CMI technologies, the raw data files and recommended readable exports are relatively small and can be readily transferred electronically. - See specific instruments for exceptions and for details about the software, data file types and recommended data exports. #### Data Sharing: - Currently, a [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) is the recommended data repository for most CMI data types, as stable specialist data repositories have not been established. #### CMI Research Resource Identifier: **RRID:SCR\_018270** - CMI users agree to [acknowledge](/resource/acknowledgments "Acknowledgments") the support of **"the Center for Macromolecular Interactions at Harvard Medical School*****"*** in publications arising from use of the facility. ### NIH Policy for Data Management and Sharing expand\_more *The National Institutes of Health (NIH) has issued its* [*Final NIH Policy for Data Management and Sharing Plans*](https://grants.nih.gov/grants/guide/notice-files/NOT-OD-21-013.html) *(DMSP) to promote the management and sharing of scientific data generated from NIH-funded or conducted research. This policy will require the submission of Data Management and Sharing Plans at the time of grant application, effective January 25, 2023.* For more information and general resources for developing a Data Management Plan in accordance with NIH guidelines, visit the [HMS Data Management Plans Page](https://datamanagement.hms.harvard.edu/plan-design/data-management-plans). **To assist with your data management plan, we have compiled information and recommendations on the common data types for CMI technologies.** ### Data Files - FPLC - AKTA Pure expand\_more Sort**Technology** **Fast Protein Liquid Chromatography (FPLC)** Instrument AKTA Pure M2 Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection and Analysis** Current Version UNICORN 7.5 (Build 7.5.01460) Data Files (Type, ~size) UNICORN data file (compressed) .res 4 MB/experiment Readable Exports data file .csv 1.5-3.5 MB/measurement image file .jpeg 60-80 KB/image ### Data Files - CD - Jasco J-1500, J-815 expand\_more SortTechnology Circular Dichroism (CD) Instruments J-1500, J-815 (retired) Recommended Repositories [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) [Protein Circular Dichroism Data Bank](https://pcddb.cryst.bbk.ac.uk/) **Software Type** Data Collection & Analysis Current Version Spectra Manager, Version 2.15.20.1 Data Files (Type, ~size) experiment file (spectrum) .jws 25 KB/expt experiment file (interval) .jwb Readable Exports exported CD spectrum .txt 5 KB/expt .csv 25 KB/expt ### Data Files - DSF - QuantStudio 6/7 expand\_more SortTechnology Differential Scanning Fluorimetry (DSF) Instrument Life Technologies Quant Studio 6/7 Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version QS Real-Time PCR Software, version 1.7.1 Data Files (Type, ~size) experiment file .eds 2-10 MB/plate **Software Type** **Data Analysis** Current Version Applied Biosystems Protein Thermal Shift, version 1.2 Data Files (Type, ~size) experiment file .eds 2-10 MB/plate Readable Exports raw data .csv 2 MB/plate analyzed data .csv 12 KB/project analyzed data .txt 29 KB/project ### Data Files - DSF - Prometheus expand\_more SortTechnology Nano Differential Scanning Fluorimetry (nanoDSF) Instrument NanoTemper Prometheus NT.Plex Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection (Thermal Denaturation)** Current Version PR.ThermControl, Version 2.3.1 Data Files (Type, ~size) experiment file .prc 10-30 MB/project raw data .xslx 2 MB/project **Software Type** **Data Analysis** Current Version PR.Stability Analysis, Version 1.1 Data Files (Type, ~size) analysis file .pra 2-6 MB/project Readable Exports processed data .xslx ~500 KB/sample results table .xslx ~30 KB/project **Software Type** **Data Collection (Chemical Denaturation)** Current Version PR.ChemControl, Version 1.4.3 Data Files (Type, ~size) experiment file .prcc 10 MB/project Readable Exports raw data .xslx 6 KB/sample **Software Type** **Data Collection (Time Control)** Current Version PR.TimeControl, Version 1.0.2 Data Files (Type, ~size) experiment file .prtime 2 MB/project Readable Exports raw data .xslx 60 KB/project ### Data Files - SEC-MALS - Dawn Heleos II expand\_more SortTechnology Size Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) Instrument Agilent 1260 Infinity LC System with variable UV detector Wyatt Dawn Heleos II MALS detector Wyatt Optilab T-rEX Refractive Index Detector Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection & Analysis** Current Version Astra, Version 7. Data Files (Type, ~size) experiment file .afe7 3-5 MB/measurement Readable Exports basic collection .csv 4 MB/measurement report .pdf 300-350 KB/measurement easi graph .csv 5 MB/experiment easi table .csv 3-5 KB/experiment ### Data Files - DLS - DynaPro Plate Reader III expand\_more SortTechnology Dynamic Light Scattering (DLS) Instrument Wyatt Dynapro Plate Reader III Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection & Analysis** Current Version Dynamics, Version 7.10.1.21 Data Files (Type, ~size) experiment file .dexp ~500KB/measurement Readable Exports Autocorrelation functions (all) .csv 20-100 KB/experiment Autocorrelation functions (indiv) .csv 10 KB/measurement Autocorrelation errors (indiv) .csv 10 KB/measurement individual acquisitions .csv 30-50 KB/measurement Well image .jpeg 350 KB/measurement Regularization fit (all) .csv 10 KB/measurement Regularization fit (indiv) .csv 10 KB/measurement peaks tables .csv 10 KB/experiment results tables .csv 5 KB/experiment ### Data Files - Mass Photometry - Refeyn TwoMP expand\_more Due to the larger file size of Mass Photometry data, the CMI instrument computer will be regularly purged of older data. Users should take extra care after each experiment to store their raw and processed data files in their own storage system. SortTechnology Mass Photometry Instrument Refeyn TwoMP Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version AccquireMP, version 2025 R1.2 Data Files (Type, ~size) processed data file (regular) .mpr ~30 MB/measurement processed data file (large) .mpr ~125 MB/measurement **Software Type** **Data Analysis** Current Version DiscoverMP, version 2025 R1 Data Files (Type, ~size) processed results (regular) .mpr ~30 MB/measurement processed results (large) .mpr ~125 MB/measurement mass calibration .mc ~1 KB/measurement workspace file .dmp ~500 bytes/experiment Readable Exports histogram .png ~200 KB/measurement events file .csv ~0.5-2 MB/measurement CMI Version History: V2025 R1 June 2025 V2024 R2 December 2024 V2024 R1.1 February 2024 V2023-R2 September 2023 V2023-R1 March 2023 V2022-R1 Initial Version ### Data Files - SPR - Biacore T200 expand\_more SortTechnology Surface Plasmanon Resonance (SPR) Instrument Biacore T200 Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version Biacore T200 Control Software, Version 2.0.1 Data Files (Type, ~size) immobilization experiment file .blr 300 KB/experiment experiment file .blr 5-20 MB/experiment method file .method 40 KB/experiment **Software Type** **Data Analysis** Current Version Biacore T200 Evaluation Software Data Files (Type, ~size) result file .bme 5-30 MB/experiment Readable Exports affinity data export .txt 3 KB/experiment kinetics data export .txt 1-2 MB/experiment results image .png 10-20 KB/experiment ### Data Files - BLI - Octet RED384 (RH16) expand\_more SortTechnology Biolayer Interferometry Instrument Octet Red 384 (now RH16) Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version Octet BLI Discovery, Version 13.0.1 Data Files (Type, ~size) experiment folder (contents below) 5 MB/experiment data file .frd 80 KB/measurement method file .fmf 35-45 KB/experiment plate definitions .jpg 30-90 KB/plate assay image .jpg 30-90 KB/experiment preprocessed data .xslx 200 KB/experiment **Software Type** **Data Analysis** Current Version Octet Analysis Studio, Version 13.0.1 Data Files (Type, ~size) HT Settings Readable Exports results table .xslx 5-10 KB Export Report .xslx CMI Version History: BLI Discovery/Analysis Studio v12 August 2021 Data Acquisition/Analysis HT v12 June 2020 Data Acquisition/Analysis HT v11 May 2018 Data Acquisition/Analysis HT v19 May 2015 ### Data Files - BLI - BLItz expand\_more SortTechnology Biolayer Interferometry Instrument Blitz Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection & Analysis** Current Version Blitz Pro 1, Version 1.2.1.5 Data Files (Type, ~size) experiment file .dataset Readable Exports data file .csv 20-150 KB/measurement image file .jpeg 40-100 KB/image ### Data Files - MST - Monolith NT.115pico expand\_more SortTechnology Microscale Thermophoresis (MST) Instrument Nanotemper Monolith NT.115pico Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version MO.Control, Version 1.6.1 Data Files (Type, ~size) experiment file .moc 1-5 MB/experiment raw data .xslx 10-25 MB/file **Software Type** **Data Analysis** Current Version MO.Affinity Analysis, Version 2.3 Data Files (Type, ~size) analysis file .nta 1 MB/experiment Readable Exports processed data .xslx 10-25 KB/file pdf report .pdf 1 MB/report ### Data Files - ITC - ITC200 expand\_more SortTechnology Isothermal Titration Calorimetry (ITC) Instrument Microcal ITC200 Recommended Repository [Generalist Repository](https://www.nlm.nih.gov/NIHbmic/generalist_repositories.html) **Software Type** **Data Collection** Current Version MicroCal iTC200, Version 1.30.0 Data Files (Type, ~size) experiment file (readable text file) .itc 250 KB/measurement **Software Type** **Data Analysis** Current Version Origin 7 (MicroCal Analysis Module), Version 7.0552 Data Files (Type, ~size) analysis file .opj 350 KB/file Readable Exports graph image .jpg 200 KB