#  DSF Services 

 



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In addition to instrument training, the CMI is now offering basic protein Differential Scanning Fluorimetry services, including protein thermal shift analysis and buffer optimization.

[CMI DSF Instruments: Life Technologies Quant Studio 6/7 and NanoTemper Technologies Prometheus NT.Plex](/differential-scanning-fluorimetry "Differential Scanning Fluorimetry (DSF)")

**DSF Services**

- protein thermal stability
- Buffer optimization



 

 [ DSF Service - Fees arrow\_circle\_right ](/differential-scanning-fluorimetry-service-fees) [ Data Collection Service - Protein Requirements arrow\_circle\_right ](/protein-requirements-cmi-services) [ DSF Instruments arrow\_circle\_right ](/differential-scanning-fluorimetry) [ About Data Collection Services arrow\_circle\_right ](/services) 

 

 

 

 



###    Sample DSF Data - Conventional with Sypro Orange  expand\_more  

DSF experiment performed on the QuantStudio 6/7 instrument using the hydrophobic dye, Sypro Orange from Life Technologies.

   ![DSF experiment](/sites/g/files/omnuum4016/files/styles/hwp_1_1__720x720_scale/public/cmi/files/dsf_pts_layout_2.jpg?itok=48YraCrJ) 

 

 

 



###    Sample DSF Data - NanoDSF, using intrinsic protein fluorescence  expand\_more  

DSF experiment performed on Prometheus NT.plex

   ![NanoDSF BSA and lysozyme](/sites/g/files/omnuum4016/files/styles/hwp_1_1__720x720_scale/public/2025-03/NanoDSF%20BSA%20lysozyme%20layout%202.jpg?itok=e64KnJg5) 

 

 

 



###    Sample DSF Buffer Screen  expand\_more  

96 unique conditions using 8 salt concentrations from 0-1 M NaCl and 12 buffers from pH 4.5 to pH 9.5, including water. Data collected on Prometheus NT.plex using protein intrinsic fluorescence at 350/330 nm.

   ![DSF buffer optimization example](/sites/g/files/omnuum4016/files/styles/hwp_1_1__720x720_scale/public/cmi/files/dsf_buffer_screen_service.jpeg?itok=NJeIstii) 

 

 

 



 

 

 

 

 



 

 See also:- [ molecular properties ](/cmi-terms/molecular-properties)