#  I want to measure a Kd, how do I choose which technique is best? 

 



The CMI has four primary technologies to quantify molecular interactions: Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI), Isothermal Titration Calorimetry (ITC) and MicroScale Thermophoresis (MST). Each technologies has its advantages and disadvantages.

Some factors to consider when choosing a binding method include:

- need for kinetic and/or equilibrium fits
- the relative size of the interactants
- the amount and concentration of samples
- the ease of labeling and/or immobilizing interactants
- the affinity of the interactants

There is no one best method for measuring molecular interactions. Request a consultation at the CMI to discuss which method might be best for your specific project.



 

 

 



 

 See also:- [ molecular interactions ](/cmi-terms/molecular-interactions)
- [ Isothermal Titration Calorimetry ](/cmi-terms/isothermal-titration-calorimetry)
- [ MicroScale Thermophoresis ](/cmi-terms/microscale-thermophoresis)
- [ Surface Plasmon Resonance ](/cmi-terms/surface-plasmon-resonance)
- [ Biolayer Interferometry ](/cmi-terms/biolayer-interferometry)