#  Light Scattering Services 

 



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In addition to instrument training, the CMI offers basic [light scattering](/light-scattering "Light Scattering") data collection services, including static light scattering to measure mass and oligomeric state and dynamic light scattering to measure hydrodynamic radius.



 

##  Light Scattering Data Collection Services 

 





###    Static Light Scattering: Mass Photometry  expand\_more  

[CMI Refeyn Two MP](/mass-photometry)

#### Standard Mass Photometry Service

For proteins and protein complexes at least 30 KDa, **without high molecular weight modifiers** (modifiers of mass >5% total).

- Detector calibration with your buffer using a protein standard
- Basic Data Analysis, in triplicate, 
    - Mass for each major peak
    - Population distribution of major peaks

#### AAV Mass Photometry Services

- For AAVs (replication-incompetent, ~3-5 MDa). *Requires Empty Capsid control*, provided by the user.
- **Empty/Full Service**
    - Detector calibration with your buffer using a protein standard
    - Basic AAV Analysis, in triplicate,
        - Empty/Full population distribution
- **AAV Extended Service**
    - Detector calibration with your buffer using a protein standard and a DNA standard on poly-lysine coated slides
    - Extended AAV Analysis, in triplicate,
        - Empty/Full population distribution
        - Mass calculated for DNA cargo

 

 



###    Static Light Scattering: SEC-MALS  expand\_more  

[CMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography](/sec-mals "Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)")

#### Standard SEC-MALS Service

**for proteins and protein complexes between 15 KDa and 1 MDa.** ***Prior purification by size-exclusion chromatography (SEC) is strongly recommended.***

***Includes:***

- Column equilibration with your buffer (pH at or below pH 7.5) or with PBS
- BSA Standard to test column performance and normalize detectors
- SEC separation of your protein sample(s)
- Basic Data analysis, including:
    - Molar Mass for each major peak
    - Hydrodynamic Radius from DLS (when light scattering signal is sufficient)

#### Additional Services

- Protein Conjugate Analysis
    - required for samples with high molecular weight modifiers, such as glycosylation or detergent micelle (modifiers of mass >5% total)
    - requires modifier dn/dc
- dn/dc measurements of detergent or protein samples

 

 



###    Dynamic Light Scattering  expand\_more  

[CMI DLS Instrument: Wyatt Dyanpro Plate Reader II](/dynamic-light-scattering "Dynamic Light Scattering (DLS)")[I](/light-scattering "Light Scattering")

#### Standard DLS Service

For proteins and biopolymers up to 1000 nm in diameter. *Includes:*

- Cumulants and Regularization fit of Hydrodynamic Radius (Rh), 1 nm - 1000 nm
- Polydispersity Analysis

#### Additional Services

- Buffer optimization

 

 



 

 

 

 

 [ Light Scattering Service - Fees arrow\_circle\_right ](/light-scattering-service-fees) [ Data Collection Service - Protein Requirements arrow\_circle\_right ](/protein-requirements-cmi-services) [ Light Scattering Instruments arrow\_circle\_right ](/light-scattering) [ About Data Collection Services arrow\_circle\_right ](/services) 

 

 

 

 

 



 

 See also:- [ molecular properties ](/cmi-terms/molecular-properties)
- [ Light Scattering ](/cmi-terms/light-scattering)