Molecular Properties/Protein Quality Services

COMING SOON!  

In the coming year, the CMI is preparing to add data collection services for assessing protein quality and sample biophysical properties.  The precise services to be offered and timeline are still in flux.

If you would like to be notified when CMI services are offered, share you opinions on which services you'd like to see provided, or to learn more, please contact the CMI.

 

Preview of CMI Data Collection Services

Light Scattering Services

SEC-MALS Service Overview

Size-exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)

CMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography

Standard SEC-MALS Service

for proteins and protein complexes between 15 KDa and 1 MDa, without high molecular weight modifiers (modifiers of mass >5% total).  Includes:

  • Column equilibration with your buffer
  • BSA Standard to test column performance and normalize detectors
  • SEC separation of your protein sample(s)
  • Basic Data analysis, including:
    • Molar Mass for each major peak
    • Hydrodynamic Radius from DLS (when light scattering signal is sufficient)

Additional Services

  • Protein Conjugate Analysis
    • required for samples with high molecular weight modifiers, such as glycosylation or detergent micelle will require additional analysis
    • requires modifier dn/dc
  • dn/dc measurements of detergent or protein samples

DLS Service Overview

Dynamic Light Scattering (DLS)

CMI DLS Instrument: Wyatt Dyanpro Plate Reader III

 

Standard DLS Service

For proteins and biopolymers up to 1000 nm in diameter. Includes:

  • Cumulants and Regularization fit of Hydrodynamic Radius (Rh), 1 nm - 1000 nm
  • Polydispersity Analysis

Additional Services

  • Aggregation Analysis - Small molecule
  • Aggregation Analysis - Protein
  • Buffer optimization

 

Light Scattering Service Fees

coming soon

DSF Services

DSF Service Overview

Differential Scanning Fluorimetry (DSF) with fluorescent dye or using intrinsic protein fluorescence

CMI DSF Instruments: Life Technologies Quant Studio 6/7 and NanoTemper Technologies Prometheus NT.Plex

  • Conventional DSF, protein thermal stability using hydrophobic dye Sypro Orange
  • NanoDSF, protein thermal stability using intrinsic protein fluorescence
  • Chemical denaturation
  • Buffer optimization by conventional DSF or NanoDSF

 

DSF Service Fees

fee table coming soon

CD Services

CD Service Overview

Circular Dichroism (CD)

CMI CD Instrument: Jasco J-815

  • Protein Far-UV Scan for Secondary Structure Analysis
    • this will strictly be a data collection service and analysis using CD deconvolution algorithms (e.g. Dichroweb) will be left to the user.
  • Protein Thermal Stability (fixed wavelength variable temperature measurement)

 

CMI Molecular Properties