#  New Shared SEC-MALS column 

 





October 04, 2016

 

 

 We've added a new shared column for [SEC-MALS](/light-scattering), an [Agilent AdvanceBio 300Å](http://www.agilent.com/cs/agilent/en-us/products/liquid-chromatography/lc-columns/biomolecule-separations/advancebio-sec).

 Column: Agilent AdvanceBio 300Å, 7.8 mm x 300 mm

- Resolution: 5KDa – 1.25 MDa (17 KDa - 667 KDa for MALS)
- pH range: 2-8.5
- Max pressure: 150 bar

 Experimental conditions

- Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 0.02% NaN3
- Sample: 100 µl BSA, 2mg/ml (Pierce 23209)
- Flowrate: 0.5 ml/min
- Runtime: 35 min
- Pressure: 67 bar

   ![SEC-MALS Data -BSA](/sites/g/files/omnuum4016/files/styles/hwp_1_1__720x720_scale/public/cmi/files/advancebio300_bsa.png?itok=2K33bqPE)