#  Protein Requirements for CMI Services 

 



CMI guidelines for protein concentration and amount for different CMI services. We recommend that users provide a concentration higher than the minimum recommended.



 

SortMethodNotesBuffer Notesminimum volume  
(μl)recommended minimum  
concentration Mass Photometry

per sample, triplicate

in storage buffer

50

Protein: ~200 nM  
(diluted to 10-20 nM)  
AAV: >1012 capsid/ml

SEC-MALS

per injection

in storage buffer

150

mg/ml > 10/mass(KDa)*  
mg/ml ~100/mass(KDa), preferred

DLS

per sample, duplicates

in storage buffer

150

mg/ml > 10/mass(KDa)*

CD

per sample

in final buffer

300

~ 0.1 mg/ml

NanoDSF

per sample, duplicates

in final buffer

50

~ 0.2 mg/ml Standard Capillaries  
~ 0.05 mg/ml High Sensitivity Capillaries

NanoDSF

buffer screen, 96 conditions

in storage buffer

200

~ 2 mg/ml

DSF, sypro

per sample, triplicates

in storage buffer

50

~ 0.5 mg/ml

DSF, sypro

buffer screen, 96 conditions

in storage buffer

200

~ 2 mg/ml

Nanobody Selection

per protein antigen, standard selection (4 rounds)

antigen buffer needs to be free of primary amines for chemical biotinylation

1 ml

~ 15 μM





\* Light Scattering Intensity is proportional to mass. A higher concentration (in mg/ml) of protein is required for small proteins than for large proteins. BSA, which is 67 KDa, and typically very pure, will give an excellent signal in MALS and DSL experiments at 2 mg/ml, and an acceptable signal at 0.2 mg/ml. For a protein that is 1/2 the mass, plan use a concentration in the 0.4-4 mg/ml range.



 

 



 

 See also:- [ molecular properties ](/cmi-terms/molecular-properties)