@article {1066321,
	title = {Genome-wide identification and characterization of Notch transcription complex-binding sequence-paired sites in leukemia cells},
	journal = {Sci Signal},
	volume = {10},
	number = {477},
	year = {2017},
	month = {2017 May 02},
	abstract = {Notch transcription complexes (NTCs) drive target gene expression by binding to two distinct types of genomic response elements, NTC monomer-binding sites and sequence-paired sites (SPSs) that bind NTC dimers. SPSs are conserved and have been linked to the Notch responsiveness of a few genes. To assess the overall contribution of SPSs to Notch-dependent gene regulation, we determined the DNA sequence requirements for NTC dimerization using a fluorescence resonance energy transfer (FRET) assay and applied insights from these in vitro studies to Notch-"addicted" T cell acute lymphoblastic leukemia (T-ALL) cells. We found that SPSs contributed to the regulation of about a third of direct Notch target genes. Although originally described in promoters, SPSs are present mainly in long-range enhancers, including an enhancer containing a newly described SPS that regulates  expression. Our work provides a general method for identifying SPSs in genome-wide data sets and highlights the widespread role of NTC dimerization in Notch-transformed leukemia cells.},
	keywords = {Animals, Enhancer Elements, Genetic, Gene Expression Profiling, Gene Expression Regulation, Leukemic, Genome, Human, Humans, Mice, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma, Promoter Regions, Genetic, Protein Binding, Receptors, Notch, Signal Transduction, Transcription Factors, Tumor Cells, Cultured},
	issn = {1937-9145},
	doi = {10.1126/scisignal.aag1598},
	author = {Severson, Eric and Arnett, Kelly L and Wang, Hongfang and Zang, Chongzhi and Taing, Len and Liu, Hudan and Pear, Warren S and Shirley Liu, X and Blacklow, Stephen C and Aster, Jon C}
}
