@article {1572174,
	title = {eIF4G has intrinsic G-quadruplex binding activity that is required for tiRNA function},
	journal = {Nucleic Acids Res},
	volume = {48},
	number = {11},
	year = {2020},
	month = {2020 06 19},
	pages = {6223-6233},
	abstract = {As cells encounter adverse environmental conditions, such as hypoxia, oxidative stress or nutrient deprivation, they trigger stress response pathways to protect themselves until transient stresses have passed. Inhibition of translation is a key component of such cellular stress responses and mounting evidence has revealed the importance of a class of tRNA-derived small RNAs called tiRNAs in this process. The most potent of these small RNAs are those with the capability of assembling into tetrameric G-quadruplex (G4) structures. However, the mechanism by which these small RNAs inhibit translation has yet to be elucidated. Here we show that eIF4G, the major scaffolding protein in the translation initiation complex, directly binds G4s and this activity is required for tiRNA-mediated translation repression. Targeting of eIF4G results in an impairment of 40S ribosome scanning on mRNAs leading to the formation of eIF2α-independent stress granules. Our data reveals the mechanism by which tiRNAs inhibit translation and demonstrates novel activity for eIF4G in the regulation of translation.},
	keywords = {Eukaryotic Initiation Factor-2, Eukaryotic Initiation Factor-4F, Eukaryotic Initiation Factor-4G, G-Quadruplexes, Humans, Peptide Chain Initiation, Translational, Phosphoproteins, Protein Biosynthesis, Protein Domains, Ribosome Subunits, Small, Eukaryotic, RNA, Messenger, RNA, Transfer},
	issn = {1362-4962},
	doi = {10.1093/nar/gkaa336},
	author = {Lyons, Shawn M and Kharel, Prakash and Akiyama, Yasutoshi and Ojha, Sandeep and Dave, Dhwani and Tsvetkov, Vladimir and Merrick, William and Ivanov, Pavel and Anderson, Paul}
}
