@article {953741,
	title = {Heterochromatin assembly by interrupted Sir3 bridges across neighboring nucleosomes},
	journal = {Elife},
	volume = {5},
	year = {2016},
	month = {2016 11 11},
	abstract = {Heterochromatin is a conserved feature of eukaryotic chromosomes with central roles in regulation of gene expression and maintenance of genome stability. Heterochromatin formation involves spreading of chromatin-modifying factors away from initiation points over large DNA domains by poorly understood mechanisms. In , heterochromatin formation requires the SIR complex, which contains subunits with histone-modifying, histone-binding, and self-association activities. Here, we analyze binding of the Sir proteins to reconstituted mono-, di-, tri-, and tetra-nucleosomal chromatin templates and show that key Sir-Sir interactions bridge only sites on different nucleosomes but not sites on the same nucleosome, and are therefore {\textquoteright}interrupted{\textquoteright} with respect to sites on the same nucleosome. We observe maximal binding affinity and cooperativity to unmodified di-nucleosomes and propose that nucleosome pairs bearing unmodified histone H4-lysine16 and H3-lysine79 form the fundamental units of Sir chromatin binding and that cooperative binding requiring two appropriately modified nucleosomes mediates selective Sir recruitment and spreading.},
	keywords = {Heterochromatin, Nucleosomes, Saccharomyces cerevisiae, Silent Information Regulator Proteins, Saccharomyces cerevisiae},
	issn = {2050-084X},
	doi = {10.7554/eLife.17556},
	author = {Behrouzi, Reza and Lu, Chenning and Currie, Mark A and Jih, Gloria and Iglesias, Nahid and Moazed, Danesh}
}
