CMI Seminar: High-Throughput Molar Mass, Size, and Interaction Measurements in Well Plates, Speaker John Champagne, Ph.D.

CMI Seminar
High-Throughput Molar Mass, Size, and Interaction Measurements in Well Plates

Speaker: John Champagne, Ph.D., Senior Applications Scientist and Northeast Regional Manager for Wyatt Technology Corp., Santa Barbara, CA USA

From aggregates to conjugates and peptides to polysaccharides, light scattering detectors combine with multiple sample preparation and delivery systems to provide a comprehensive suite of biophysical characterization tools. The light scattering toolkit can determine molar mass, size, charge, interactions, conformation and conjugation of macromolecules and nanoparticles.

This seminar presents an overview of the instrumentation and examples of the tasks relevant to well-characterized biopharmaceuticals that may be accomplished with these tools, focusing on the one central techniques of HT-DLS. In this seminar, the applications of the DynaPro for aggregation and stability studies will be presented.

• Aggregation and stability are important criteria for selecting biotherapeutic candidates for developability and optimizing their formulation. Screening of the broadest matrix of conditions and excipients enables true QbD, imparting confidence in the ultimate choice of candidates and formulations with which to move forward.
• The DynaPro Plate Reader IIIcontributes major productivity enhancements in a biopharmaceutical setting, greatly increasing the speed of analysis without sacrificing sensitivity.
• The DynaPro Plate Reader IIIbrings dynamic light scattering (DLS) to the realm of high-throughput, automated measurements of size, eliminating tedious cuvette-based measurements.