Biolayer Interferometry (BLI)

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip.  BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time. 

The CMI has two ForteBio instruments for measuring BLI: the Octet RED384 and the BLItz.  The Octet RED384 is more sensitive and higher throughput and can be used for macromolecular and small molecule binding experiments.  The BLItz uses a very small sample volume, but is more time consuming and cannot measure molecules smaller than 10 KDa.

 

For information on access fees, policies and getting started at the CMI, see the CMI Access Page.

 

Required supplies for Octet:

  • ForteBio Dip and Read sensors
  • Black 96-well plate to soak sensors
  • Black 96-well or 384-well plate for samples and reagents

 

Required supplies for BLItz:

  • ForteBio Dip and Read sensors
  • 96-well plate to soak sensors
  • Black 0.5 ml tubes (provided by CMI)

 

ForteBio Dip and Read Biosensors:

  • Streptavidin (SA) biosensors, part number 18-5019
  • Super-Streptavidin (SSA) biosensors, part number 18-5057
  • anti-His (HIS1K) biosensors, NEW -for kinetics, part number 18-5120
  • Ni-NTA (NTA) biosensors, part number 18-5101
  • anti-GST biosensors, part number 18-5096
  • See ForteBio website for more sensor types

Plates:

  • Greiner Bio-One** 96-well black flat-bottom PP, part number 655209 (VWR 82050-784)
  • Greiner Bio-One 384-well black flat-bottom PP, part number 781209 (VWR 82051-318)
  • ForteBio 384-well black tilted-bottom PP, part number 18-5080
** Only Greiner Bio-One brand, black microplates or ForteBio plates are recommended by ForteBio for the Octet sample and reagent plate.