Light Scattering Services
In addition to instrument training, the CMI offers basic light scattering data collection services, including static light scattering to measure mass and oligomeric state and dynamic light scattering to measure hydrodynamic radius.
Light Scattering Data Collection Services
Standard Mass Photometry Service
For proteins and protein complexes at least 30 KDa, without high molecular weight modifiers (modifiers of mass >5% total).
- Detector calibration with your buffer using a protein standard
- Basic Data Analysis, in triplicate,
- Mass for each major peak
- Population distribution of major peaks
AAV Mass Photometry Services
- For AAVs (replication-incompetent, ~3-5 MDa). Requires Empty Capsid control, provided by the user.
- Empty/Full Service
- Detector calibration with your buffer using a protein standard
- Basic AAV Analysis, in triplicate,
- Empty/Full population distribution
- AAV Extended Service
- Detector calibration with your buffer using a protein standard and a DNA standard on poly-lysine coated slides
- Extended AAV Analysis, in triplicate,
- Empty/Full population distribution
- Mass calculated for DNA cargo
CMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography
Standard SEC-MALS Service
for proteins and protein complexes between 15 KDa and 1 MDa. Prior purification by size-exclusion chromatography (SEC) is strongly recommended.
Includes:
- Column equilibration with your buffer (pH at or below pH 7.5) or with PBS
- BSA Standard to test column performance and normalize detectors
- SEC separation of your protein sample(s)
- Basic Data analysis, including:
- Molar Mass for each major peak
- Hydrodynamic Radius from DLS (when light scattering signal is sufficient)
Additional Services
- Protein Conjugate Analysis
- required for samples with high molecular weight modifiers, such as glycosylation or detergent micelle (modifiers of mass >5% total)
- requires modifier dn/dc
- dn/dc measurements of detergent or protein samples
CMI DLS Instrument: Wyatt Dyanpro Plate Reader III
Standard DLS Service
For proteins and biopolymers up to 1000 nm in diameter. Includes:
- Cumulants and Regularization fit of Hydrodynamic Radius (Rh), 1 nm - 1000 nm
- Polydispersity Analysis
Additional Services
- Buffer optimization