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Light Scattering Services

Light Scattering Data Collection Services

SEC-MALS Service Overview

Size-exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)

CMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography

Standard SEC-MALS Service

for proteins and protein complexes between 15 KDa and 1 MDa, without high molecular weight modifiers (modifiers of mass >5% total).  Prior purification by size-exclusion chromatography (SEC) is strongly recommended.  

Includes:

  • Column equilibration with your buffer (pH at or below pH 7.5) or with PBS
  • BSA Standard to test column performance and normalize detectors
  • SEC separation of your protein sample(s)
  • Basic Data analysis, including:
    • Molar Mass for each major peak
    • Hydrodynamic Radius from DLS (when light scattering signal is sufficient)

Additional Services

  • Protein Conjugate Analysis
    • required for samples with high molecular weight modifiers, such as glycosylation or detergent micelle will require additional analysis
    • requires modifier dn/dc
  • dn/dc measurements of detergent or protein samples

SEC-MALS example data - BSA

Column: Agilent AdvanceBio 300Å, 7.8 mm x 300 mm

  • Resolution: 5KDa – 1.25 MDa (17 KDa - 667 KDa for MALS)
  • pH range: 2-8.5
  • Max pressure: 150 bar

Experimental conditions

  • Buffer:  25 mM HEPES pH 7.5, 150 mM NaCl, 0.02% NaN3
  • Sample: 100 µl BSA, 2mg/ml (Pierce 23209)
  • Flowrate: 0.5 ml/min
  • Runtime: 35 min
  • Pressure: 67 bar

SEC-MALS Data -BSA

DLS Service Overview

Dynamic Light Scattering (DLS)

CMI DLS Instrument: Wyatt Dyanpro Plate Reader III

 

Standard DLS Service

For proteins and biopolymers up to 1000 nm in diameter. Includes:

  • Cumulants and Regularization fit of Hydrodynamic Radius (Rh), 1 nm - 1000 nm
  • Polydispersity Analysis

Additional Services

  • Aggregation Analysis - Small molecule
  • Buffer optimization

 

Light Scattering Data Collection Fees Accordion

Data Collection Fees Summary

Data Collection Fees

  • Limited Data Collection Services are offered. Service fees will be charged for all completed services, regardless of experimental outcome. 
  • Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
    • External Users will also be required to submit a PO and a signed CMI User Agreement.
  • Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee. Some services include one or more replicate measurements by default in the sample fee (e.g. DLS and DSF). For others, there may be reduced-price replicate measurement fee (e.g. SEC-MALS and CD) if collected in the same dataset.

Data Collection Fee List

Light Scattering - SEC-MALS Data Collection Fees

 

Harvard
base price

Non-Harvard
(+F&A)

Commercial

Life Lab

SEC-MALS -setup

 per buffer, includes analytical column equilibration, BSA control

$425

$518.50

$1,037.00

$777.75

SEC-MALS data collection

 per sample, analytical column, 0.5 ml/min

$250

$305.00

$610.00

$457.50

SEC-MALS replicate injection

additional injection collected in the same sequence

$100 $122 $244 $183

dn/dc measurement

 per user-provided reagent

$445

$542.90

$1,085.80

$814.35

SEC PBS Buffer preparation 

 up to 1 L, filtered and degassed

$50

$61

$122

$91.50

SEC Custom Buffer preparation 

 up to 1 L, made using reagents available at the CMI (HEPES, Phosphate, NaCl, reducing agents)

$150 $183 $366 $274.50

DLS analysis - plate setup

per set, up to 48 samples

$250 $305 $610 $457.50

DLS data collection -macromolecules

per sample, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well

$60

$73.20 $146.40 $109.80
         

 

Light Scattering - DLS Data Collection Fees

 

Harvard
base price

Non-Harvard (+F&A)

Commercial

Life Lab

DLS analysis - plate setup

per set, up to 48 samples

$250

$305.00

$610.00

$457.50

DLS data collection -macromolecules

per sample, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well

$60

$73.20

$146.40

$109.80

Example Project Fees

 

Example project details: Light Scattering analysis project for SEC-MALS of 2 proteins in one buffer system. Project setup includes column equilibration (12+ column volumes) into user-provided buffer and a BSA control experiment.  One additional replicate injection of each sample was requested.

line item Qty Internal price External Academic price
SEC-MALS setup  1 $425 $518.50
SEC-MALS data collection 2 2x $250 2x $305.00
SEC-MALS replicate injection 2 2x $100  2x $122.00
Total   $1,125 $1372.50