CMI SEC-MALS System: Wyatt Dawn Heleos with in-line DLS, RI UV, Agilent chromatography
Column: Agilent AdvanceBio 300Å, 7.8 mm x 300 mm
Experimental conditions
CMI DLS Instrument: Wyatt Dyanpro Plate Reader III
For proteins and biopolymers up to 1000 nm in diameter. Includes:
Harvard |
Non-Harvard |
Commercial |
Life Lab |
|
SEC-MALS -setup per buffer, includes analytical column equilibration, BSA control |
$425 |
$518.50 |
$1,037.00 |
$777.75 |
SEC-MALS data collection per sample, analytical column, 0.5 ml/min |
$250 |
$305.00 |
$610.00 |
$457.50 |
SEC-MALS replicate injection additional injection collected in the same sequence |
$100 | $122 | $244 | $183 |
dn/dc measurement per user-provided reagent |
$445 |
$542.90 |
$1,085.80 |
$814.35 |
SEC PBS Buffer preparation up to 1 L, filtered and degassed |
$50 |
$61 |
$122 |
$91.50 |
SEC Custom Buffer preparation up to 1 L, made using reagents available at the CMI (HEPES, Phosphate, NaCl, reducing agents) |
$150 | $183 | $366 | $274.50 |
DLS analysis - plate setup per set, up to 48 samples |
$250 | $305 | $610 | $457.50 |
DLS data collection -macromolecules per sample, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well |
$60 |
$73.20 | $146.40 | $109.80 |
Harvard |
Non-Harvard (+F&A) |
Commercial |
Life Lab |
|
DLS analysis - plate setup per set, up to 48 samples |
$250 |
$305.00 |
$610.00 |
$457.50 |
DLS data collection -macromolecules per sample, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well |
$60 |
$73.20 |
$146.40 |
$109.80 |
Example project details: Light Scattering analysis project for SEC-MALS of 2 proteins in one buffer system. Project setup includes column equilibration (12+ column volumes) into user-provided buffer and a BSA control experiment. One additional replicate injection of each sample was requested.
line item | Qty | Internal price | External Academic price |
---|---|---|---|
SEC-MALS setup | 1 | $425 | $518.50 |
SEC-MALS data collection | 2 | 2x $250 | 2x $305.00 |
SEC-MALS replicate injection | 2 | 2x $100 | 2x $122.00 |
Total | $1,125 | $1372.50 |
In 2022, the CMI launched data collection services for assessing protein quality and sample biophysical properties. To start a conversation about a data collection services project, please contact the CMI or send a Sample Form to cmi@hms.harvard.edu.