The CMI offers Training/Access on SEC-MALS and DLS instruments and Data Collection Services.
Size exclusion chromatography with multi-angle static light scattering (SEC-MALS) is used to accurately measure weight-averaged masses (Mw) of macromolecules in solution by measure the intensity of scattered light of a sample as it elutes from an SEC column.
Dynamic Light Scattering (DLS) measures the time-dependent fluctuations in scattered light. These fluctuations are directly related to rate of diffusion of the molecule through the solvent, and can be used to measure hydrodynamic radius (Rh) of the particles in solution.
Mass Photometry (MP) is a single-particle light scattering method used to measure mass of macromolecules in solution, >30 KDa and for which standards with a shared refractive index are known (e.g. Protein or DNA).