molecular properties
Content tagged with molecular properties
May I collect data during training?
We do NOT collect User Data during CMI training sessions.
All Training sessions include:
- Overview of the instrument and maintenance procedures
- Tutorial on control and analysis software
- Review of experimental design
- User trainings may be individual or small...
Where do I get supplies? *
Most specialized instrument supplies and reagents will be purchased by the user directly from the vendor
Including:
- BLI: Sartorius Biosensors
- SPR: Cytiva Sensor Chips (Biacore SERIES S)
- SPR: Cytiva Immobilization reagents
- MP: Refeyn pre-cleaned cover slides
- Nan...
Mass Photometry (MP)
The TwoMP from Refeyn for Mass Photometry is a single-particle static light scattering detector, used to measure molar mass in solution.
How pure does my protein sample need to be for analysis by DSF, CD or light scattering?
The more pure your sample is, the easier it is to interpret the results, as contaminating proteins may contribute to your overall signal in bulk measurements.
Differential Scanning Fluorimetry (DLS), Circular Dichroism (CD) and Dynamic Light Scattering...
How much protein is needed for molecular property analysis by DSF, CD, or light scattering?
The amount of protein needed will vary by method and sometimes by the mass of protein to be characterized. See CMI Service Protein Requirements for estimates and recommendations for the amount of protein needed for standard data collection.
Protein Requirements for CMI Services
Are there fluorescent dyes than can be used for Differential Scanning Fluorimetry (DSF) of Membrane Proteins?
If your membrane protein has fluorescent residues (tryptophan and tyrosine), you should consider trying our Prometheus NT.plex for DSF using intrinsic protein fluorescence (NanoDSF).
However, if you wish to do conventional DSF (dye-based), please note that...