CD at the CMI
Circular Dichroism (CD) Spectroscopy is used to determine the optical isomerism and secondary structure of molecules. Circular dichroism (measured in molar ellipticity) is the difference in absorption of left-handed and right-handed circularly polarized light and can be observed in optically active molecules with chiral centers. Proteins have many chiral centers. CD spectra in the Far-UV region (185 – 250 nm) can be used to determine protein secondary structure. Characteristic peaks for Thermal stability (Tm) can be measured by following changes in molar ellipticity with increasing temperature.
The CMI has a Jasco J-815 CD Spectropolarimeter with a Peltier temperature controller and single cuvette holder.