Circular Dichroism (CD) Spectroscopy is used to determine the optical isomerism and secondary structure of molecules. Circular dichrosim (measured in molar ellipticity) is the difference in absorption of left-handed and right-handed circularly polarized light and can be observed in molecules with chiral centers. CD spectra in the "far UV" region (185-250 nm) can be used to determine protein secondary structure. Thermal stability (Tm) can be measured by following changes in molar ellipticity with increasing temperature.
The CMI has a Jasco J-815 CD Spectropolarimeter with a Peltier temperature controller and single cuvette holder.
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