The National Institutes of Health (NIH) has issued its Final NIH Policy for Data Management and Sharing Plans (DMSP) to promote the management and sharing of scientific data generated from NIH-funded or conducted research. This policy will require the submission of Data Management and Sharing Plans at the time of grant application, effective January 25, 2023. For more information and general resources for developing a Data Management Plan in accordance with NIH guidelines, visit the HMS Data Management Plans Page.
Data Management
About CMI Data Files
Users are responsible for storage of all raw and processed data collected at the CMI.
- Users should have a plan to copy or transfer all raw and process data to their own local or cloud storage system.
- While the CMI allows temporary local storage of CMI User data on the instrument computer, we make no guarantees on the security or long-term availability of any data at the CMI.
- For most (but not all) CMI technologies, the raw data files and recommended readable exports are relatively small and can be readily transferred electronically.
- See specific instruments for exceptions and for details about the software, data file types and recommended data exports.
Data Sharing:
- Currently, a Generalist Repository is the recommended data repository for most CMI data types, as stable specialist data repositories have not been established.
Data Files - CD - Jasco J-815, Jasco J-1500
| Technology | Circular Dichroism (CD) | ||
| Instruments | Jasco J-815, J-1500 | ||
| Recommended Repository |
Generalist Repository Protein Circular Dichroism Data Bank |
||
| Software Type | Data Collection & Analysis | ||
| Current Version | Spectra Manager, Version 2.15.20.1 | ||
| Data Files (Type, ~size) |
experiment file (spectrum) experiment file (interval) |
.jws .jwb |
25 KB/expt |
| Readable Exports | exported CD spectrum |
.txt .cvs |
5 KB/expt 25 KB/expt |
Data Files - DSF - QuantStudio 6/7
| Technology | Differential Scanning Fluorimetry (DSF) | ||
| Instrument | Life Technologies Quant Studio 6/7 | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | QS Real-Time PCR Software, version 1.7.1 | ||
| Data Files (Type, ~size) | experiment file | .eds | 2-10 MB/plate |
| Software Type | Data Analysis | ||
| Current Version | Applied Biosystems Protein Thermal Shift, version 1.2 | ||
| Data Files (Type, ~size) | experiment file | .eds | 2-10 MB/plate |
| Readable Exports | raw data | .csv | 2 MB/plate |
| analyzed data | .csv | 12 KB/project | |
| analyzed data | .txt | 29 KB/project | |
Data Files - DSF - Prometheus
| Technology | Nano Differential Scanning Fluorimetry (nanoDSF) | ||
| Instrument | NanoTemper Prometheus NT.Plex | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection (Thermal Denaturation) | ||
| Current Version | PR.ThermControl, Version 2.3.1 | ||
| Data Files (Type, ~size) | experiment file | .prc | 10-30 MB/project |
| raw data | .xslx | 2 MB/project | |
| Software Type | Data Analysis | ||
| Current Version | PR.Stability Analysis, Version 1.1 | ||
| Data Files (Type, ~size) | analysis file | .pra | 2-6 MB/project |
| Readable Exports | processed data | .xslx | ~500 KB/sample |
| results table | .xslx | ~30 KB/project | |
| Software Type | Data Collection (Chemical Denaturation) | ||
| Current Version | PR.ChemControl, Version 1.4.3 | ||
| Data Files (Type, ~size) | experiment file | .prcc | 10 MB/project |
| Readable Exports | raw data | .xslx | 6 KB/sample |
| Software Type | Data Collection (Time Control) | ||
| Current Version | PR.TimeControl, Version 1.0.2 | ||
| Data Files (Type, ~size) | experiment file | .prtime | 2 MB/project |
| Readable Exports | raw data | .xslx | 60 KB/project |
Data Files - SEC-MALS - Dawn Heleos II
| Technology | Size Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) | ||
| Instrument |
Agilent 1260 Infinity LC System with variable UV detector Wyatt Dawn Heleos II MALS detector Wyatt Optilab T-rEX Refractive Index Detector |
||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection & Analysis | ||
| Current Version | Astra, Version 7. | ||
| Data Files (Type, ~size) | experiment file | .afe7 | 3-5 MB/measurement |
| Readable Exports | basic collection | .csv | 4 MB/measurement |
| report | 300-350 KB/measurement | ||
| easi graph | .csv | 5 MB/experiment | |
| easi table | .csv | 3-5 KB/experiment | |
Data Files - DLS - DynaPro Plate Reader III
| Technology | Dynamic Light Scattering (DLS) | ||
| Instrument | Wyatt Dynapro Plate Reader III | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection & Analysis | ||
| Current Version | Dynamics, Version 7.10.1.21 | ||
| Data Files (Type, ~size) | experiment file | .dexp | ~500KB/measurement |
| Readable Exports | Autocorrelation functions (all) | .csv | 20-100 KB/experiment |
| Autocorrelation functions (indiv) | .csv | 10 KB/measurement | |
| Autocorrelation errors (indiv) | .csv | 10 KB/measurement | |
| individual acquisitions | .csv | 30-50 KB/measurement | |
| Well image | .jpeg | 350 KB/measurement | |
| Regularization fit (all) | .csv | 10 KB/measurement | |
| Regularization fit (indiv) | .csv | 10 KB/measurement | |
| peaks tables | .csv | 10 KB/experiment | |
| results tables | .csv | 5 KB/experiment | |
Data Files - Mass Photometry - Refeyn TwoMP
Due to the large filesize of Mass Photometry data, the CMI instrument computer will be regularly purged of older data. Users should take extra care after each experiment to store their raw and processed data files in their own storage system.
|
Technology |
Mass Photometry | ||
| Instrument | Refeyn TwoMP | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | AccquireMP, version 2024 R1.1 | ||
| Data Files (Type, ~size) | processed data file (regular) | .mpr | ~30 MB/measurement |
| processed data file (large) | .mpr | ~125 MB/measurement | |
| Software Type | Data Analysis | ||
| Current Version | DiscoverMP, version 2024 R1 | ||
| Data Files (Type, ~size) | processed results (regular) | .mpr | ~30 MB/measurement |
| processed results (large) | .mpr | ~125 MB/measurement | |
| mass calibration | .mc | ~1 KB/measurement | |
| workspace file | .dmp | ~500 bytes/experiment | |
| Readable Exports | histogram | .png | ~200 KB/measurement |
| events file | .csv | ~0.5-2 MB/measurement | |
CMI Version History:
V2023-R2 September 2023
V2023-R1 March 2023
V2022-R1 Initial Version
Data Files - SPR - Biacore T200
| Technology | Surface Plasmanon Resonance (SPR) | ||
| Instrument | Biacore T200 | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | Biacore T200 Control Software, Version 2.0.1 | ||
| Data Files (Type, ~size) | immobilization experiment file | .blr | 300 KB/experiment |
| experiment file | .blr | 5-20 MB/experiment | |
| method file | .method | 40 KB/experiment | |
| Software Type | Data Analysis | ||
| Current Version | Biacore T200 Evaluation Software | ||
| Data Files (Type, ~size) | result file | .bme | 5-30 MB/experiment |
| Readable Exports | affinity data export | .txt | 3 KB/experiment |
| kinetics data export | .txt | 1-2 MB/experiment | |
| results image | .png | 10-20 KB/experiment | |
Data Files - BLI - Octet RH16 (RED384)
| Technology | Biolayer Interferometry | ||
| Instrument | Octet RH16 (Octet Red 384) | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | Octet BLI Discovery, Version 13.0 | ||
| Data Files (Type, ~size) | experiment folder (contents below) | 5 MB/experiment | |
| data file | .frd | 80 KB/measurement | |
| method file | .fmf | 35-45 KB/experiment | |
| plate definitions | .jpg | 30-90 KB/plate | |
| assay image | .jpg | 30-90 KB/experiment | |
| preprocessed data | .xslx | 200 KB/experiment | |
| Software Type | Data Analysis | ||
| Current Version | Octet Analysis Studio, Version 13.0 | ||
| Data Files (Type, ~size) | HT Settings | ||
| Readable Exports | results table | .xslx | 5-10 KB |
| Export Report | .xslx | ||
Data Files - BLI - BLItz
| Technology | Biolayer Interferometry | ||
| Instrument | Blitz | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection & Analysis | ||
| Current Version | Blitz Pro 1, Version 1.2.1.5 | ||
| Data Files (Type, ~size) | experiment file | .dataset | |
| Readable Exports | data file | .csv | 20-150 KB/measurement |
| image file | .jpeg | 40-100 KB/image | |
Data Files - MST - Monolith NT.115pico
| Technology | Microscale Thermophoresis (MST) | ||
| Instrument | Nanotemper Monolith NT.115pico | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | MO.Control, Version 1.6.1 | ||
| Data Files (Type, ~size) | experiment file | .moc | 1-5 MB/experiment |
| raw data | .xslx | 10-25 MB/file | |
| Software Type | Data Analysis | ||
| Current Version | MO.Affinity Analysis, Version 2.3 | ||
| Data Files (Type, ~size) | analysis file | .nta | 1 MB/experiment |
| Readable Exports | processed data | .xslx | 10-25 KB/file |
| pdf report | 1 MB/report | ||
Data Files - ITC - ITC200
| Technology | Isothermal Titration Calorimetry (ITC) | ||
| Instrument | Microcal ITC200 | ||
| Recommended Repository | Generalist Repository | ||
| Software Type | Data Collection | ||
| Current Version | MicroCal iTC200, Version 1.30.0 | ||
| Data Files (Type, ~size) | experiment file (readable text file) | .itc | 250 KB/measurement |
| Software Type | Data Analysis | ||
| Current Version | Origin 7 (MicroCal Analysis Module), Version 7.0552 | ||
| Data Files (Type, ~size) | analysis file | .opj | 350 KB/file |
| Readable Exports | graph image | .jpg | 200 KB |