MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions with a wide range of affinities (pM-mM). The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge or size. Since one or all of these parameters changes with virtually every binding event, a wide range of biomolecules can be measured from ions and small molecule fragments to large macromolecular complexes in very small volumes (<10 ul) in a wide range of standard buffers and complex mixtures including liposomes, detergent, serum, and cell lysates.
The CMI has a Monolith NT.115pico from NanoTemper Technologies.
For information on access fees, policies and getting started at the CMI, see the CMI Access Page.