The Biacore T200 is a instrument that measures Surface Plasmon Resonance (SPR) and is used to measure molecular interactions in real time. In an SPR experiment, one molecule (the Ligand) is immobilized to a sensor chip and binding to a second molecule (the Analyte) is measured under flow. Response is measured in resonance units (RU) and is proportional to the mass on the surface and for any given interactant, the response is proportional to the number of molecules bound to the surface. Response is recorded and displayed on a sensogram in real time. SPR experiments can be used to determine the kinetics (association and dissociation rate constants, kon and koff) and affinity (equilibrium binding constants, KA and KD) of molecular interactions.
The CMI has a Biacore T200 from GE.
For information on access fees, policies and getting started at the CMI, see the CMI Access Page.