The Center for Macromolecular Interactions
Center for Macromolecular Interactions
The Center for Macromolecular Interactions (CMI) is primarily a shared instrument facility offering training and access on our technologies. The CMI has a mission to enhance basic research in the HMS community by providing scientific consultation, training and access to shared biophysical instruments for the characterization and analysis of macromolecules and their complexes.
Molecular Properties
The CMI offers training and access to instruments to measure molecular properties, including Differential Scanning Fluorimetry (DSF), Circular Dichroism (CD), Light Scattering, including size-exclusion chromatography with multi-angle light scattering (SEC-MALS), Dynamic Light Scattering (DLS), Mass Photometry (MP), and protein production, including Fast Protein Liquid Chromatography (FPLC) and Cell-free Protein Synthesis. The CMI also offers limited data collection services on select CMI technologies, as staff and instrument time allows.
Molecular Interactions
The CMI also offers training and access to instruments to measure molecular interactions, including Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR), Biolayer Interferometry (BLI), MicroScale Thermophoresis (MST), and a Multimode Plate Reader for Fluorescent and Luminescent assays.
CMI Events
Seminar - Refeyn MassFluidix HC add-on for Mass Photometry
CMI News
Octet R8e for Biolayer Interferometry Installed
The new Octet R8e, has improved sensitivity and extended temperature control.
CMI Binding Simulator
CMI Rates for FY27 Posted
Newest Technologies at the CMI
Biolayer Interferometry (BLI)
The Octet R8e and RED384 (RH16) instruments from Sartorius detect Biolayer Interferometry, an optical technique used to measure binding kinetics and binding equilibrium.
Mass Photometry (MP)
The TwoMP from Refeyn for Mass Photometry is a single-particle static light scattering detector, used to measure molar mass in solution.
Multimode Plate Reader
Spark Multimode Plate Reader from Tecan, configured with Absorbance mode, Luminescence mode, and enhanced Fusion Optics Fluorescence mode.
Cell-Free Protein Synthesis
The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip.
Fast Protein Liquid Chromatography (FPLC)
The AKTA Pure from Cytiva is a preparative liquid chromatography system, used for protein purification by Size-Exclusion Chromatography and Ion Exchange.
Recent CMI User Publications
- CMI users agree to acknowledge the support of "the Center for Macromolecular Interactions at Harvard Medical School" in publications arising from use of the facility. CMI RRID: SCR_018270.