Center for Macromolecular Interactions
The Center for Macromolecular Interactions (CMI) in the department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School has a mission to enhance basic research in the HMS community by providing scientific consultation, training and access to shared biophysical instruments for the characterization and analysis of macromolecules and their complexes.
The facility currently offers training and access to instruments for Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR), Biolayer Interferometry (BLI), MicroScale Thermophoresis (MST), Differential Scanning Fluorimetry (DSF), Circular Dichroism (CD), and Light Scattering: including size-exclusion chromatography with multi-angle light scattering (SEC-MALS), Dynamic Light Scattering (DLS), and Mass Photometry (MP).
The CMI offers data collection services for the characterization of protein secondary structure, mass and oligomeric state, polydispersity, aggregation state, hydrodynamic radius, and thermal stability. Using a library developed in the lab of Andrew Kruse, the CMI is also offering yeast surface display nanobody selections services.
Recent CMI User Publications
We are pleased to announce the installation of our newest instrument, the Refeyn TwoMP instrument for Mass Photometry, allowing single-particle mass measurements in solution. Thanks to the HMS Foundry for helping to fund this new equipment purchase!
We will begin training in December. Current CMI users interested in using mass photometry should make a training request in PPMS. Interested new users should request an account at the CMI.
The CMI is now offering protein data collection services, including light scattering by SEC-MALS and DLS, differential scanning fluorimetry and circular dichroism, as well as nanobody production services using yeast surface display libraries developed in the lab of Andrew Kruse.