Center for Macromolecular Interactions
Welcome to the Center for Macromolecular Interactions (CMI) in the department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School. Our mission is to enhance basic research in the HMS community by providing scientific consultation, training and access to shared biophysical instruments for the characterization and analysis of macromolecules and their complexes.
The facility currently offers training and access to instruments for Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR), Biolayer Interferometry (BLI), Differential Scanning Fluorimetry (DSF), Circular Dichroism (CD), Light Scattering: size-exclusion chromatography with multi-angle light scattering (SEC-MALS) and Dynamic Light Scattering (DLS), and MicroScale Thermophoresis (MST).
The CMI offers data collection services for the characterization of protein secondary structure, mass and oligomeric state, polydispersity, aggregation state, hydrodynamic radius, and thermal stability. Using a library developed in the lab of Andrew Kruse, the CMI is also offering yeast surface display nanobody selections services.
Recent CMI User Publications
The CMI is now offering protein data collection services, including light scattering by SEC-MALS and DLS, differential scanning fluorimetry and circular dichroism, as well as nanobody production services using yeast surface display libraries developed in the lab of Andrew Kruse.