Active and Potential CMI users and labs may request a letter of support for grant applications, from the CMI Core director, Kelly Arnett (cmi @hms.harvard.edu).
- New users should contact the CMI to discuss you project and how to get started at the CMI.
- Existing CMI user labs may use the description of CMI resources below or request a personalized letter of support.
General Description of CMI Resources
The Center for Macromolecular Interactions at Harvard Medical School (CMI) provides scientific consultation, training and access to shared biophysical equipment for the characterization of macromolecules and their complexes. Instruments at the CMI include a Microcal ITC200 for Isothermal Titration Calorimetry (ITC), a GE Biacore T200 for Surface Plasmon Resonance (SPR), a ForteBio Octet RED384 for Biolayer Interferometry (BLI), a NanoTemper Technologies Monolith NT.115pico for MicroScale Thermophoresis (MST), a Life Technologies Quant Studio 6 and a NanoTemper Prometheus NT.Plex for protein thermal shift analysis by Differential Scanning Fluorimetry (DSF), a Jasco J-1500 spectropolarimeter for Circular Dichroism (CD), an analytical Size Exclusion Chromatography system with Wyatt Dawn Heleos II Multi-Angle Light Scattering detector for SEC-MALS, a Wyatt DynaPro Plate Reader III for Dynamic Light Scattering (DLS) and a Refeyn TwoMP for Mass Photometry. These instruments provide several orthogonal methods for measuring protein/protein and protein/ligand interactions and for characterizing protein quality. Since launch of the CMI in early 2015, over 170 scientific publications in high impact journals have featured work performed at the CMI (cmi.hms.harvard.edu/publications). To offset some of the costs associated with instrument maintenance and operation, all CMI users are charged fees for instrument access and training. CMI fees are reevaluated biannually and published on the website (cmi.hms.harvard.edu/fees).
Please let us know if you get a grant using or planning CMI services!