Data Collection Service Fees

FY23 Fees - Data Collection (effective from July 1, 2022)

Data Collection Fees Summary

Data Collection Fees

  • Limited Data Collection Services are offered. Service fees will be charged for all completed services, regardless of experimental outcome. 
  • Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
    • External Users will also be required to submit a PO and a signed CMI User Agreement.
  • Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee. Some services include one or more replicate measurements by default in the sample fee (e.g. DLS and DSF). For others, there may be reduced-price replicate measurement fee (e.g. SEC-MALS and CD) if collected in the same dataset.

Data Collection Fee List

Light Scattering Data Collection Fees

  Harvard base price Non-Harvard (+F&A) Commercial Life Lab
DLS - plate setup $270.00 $329.40 $658.80 $494.10
Setup fee per plate, up to 48 samples, includes BSA control and 1 buffer control. In addition to per-sample fee.
DLS - sample data collection - macromolecules $75.00 $91.50 $183.00 $137.25
Per sample fee, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well, in addition to plate setup fee.
SEC-MALS -column setup  $450.00 $549.00 $1,098.00 $823.50
per buffer, includes analytical column equilibration, BSA control, in addtion to per-sample fee.
SEC-MALS data collection $250.00 $305.00 $610.00 $457.50
per sample, analytical column, 0.5 ml/min
SEC-MALS replicate injection  $110.00 $134.20 $268.40 $201.30
replicate sample injection collected in the same sequence
dn/dc measurement $455.00 $555.10 $1,110.20 $832.65
per user-provided reagent
SEC PBS Buffer preparation $65.00 $79.30 $158.60 $118.95
up to 1 L PBS buffer, filtered and degassed
SEC Custom Buffer preparation $160.00 $195.20 $390.40 $292.80
up to 1 L custom buffer, made using reagents available at the CMI (HEPES, Phosphate, NaCl, reducing agents)

DSF Data Collection Fees

  Harvard Non-Harvard (+F&A) Commercial Life Lab
base price
DSF thermal shift analysis -plate setup  $        275  $   335.50  $     671.00 503.25
Setup fee per plate, up to 30 samples, includes lysozyme control and 1 buffer control. DSF on the QuantStudio using SyproOrange protein stain. In addition to per-sample fee.
DSF sample data collection  $           70  $     85.40  $     170.80 128.1
Per sample fee, measured in triplicate, 2C/min, in addition to setup fee.
DSF buffer optimization screen  $        455  $   555.10  $  1,110.20 832.65
96 conditions NaCl vs pH, 1 measurement each, 2C/min
nanoDSF thermal shift analysis -setup  $        275  $   335.50  $     671.00 $     503.25
Setup fee per capillary set, up to 10 samples, includes lysozyme or BSA control. DSF on the Prometheus using protein fluorescence. In addition to per-sample fee.
nanoDSF sample data collection  $           70  $     85.40  $     170.80 $     128.10
Per sample fee, measured in duplicate, 2C/min, in addition to setup fee.
Per sample fee, measured in duplicate, 2C/min, PREMIUM CAPILLARIES (High Sensitivity Capillaries for low concentration) $           110 $     134.20 $     268.40 $     201.30
nanoDSF Buffer Optimization Screen  $        595  $   725.90  $  1,451.80 $   1088.85
96 conditions NaCl vs pH, 1 measurement each, 2C/min

 

CD Data Collection Fees

  Harvard base price Non-Harvard (+F&A) Commercial Life Lab
CD scan set up fee $195 $237.90 $475.80 $356.85
Setup fee per dataset (up to 20 scans), includes 1 buffer control. Additional buffers should be listed as samples.
CD scan for secondary structure $135 $164.70 $329.40 $247.05
Per sample, Far-UV 260-185 nm scan, 5-10 accumulations, in addition to setup fee.
CD replicate sample scan $70 $73.20 $146.40 $109.80
Replicate sample collected in the same dataset (or alternate temperature scan of existing sample)
CD thermal melt $510 $622.20 $1,244.40 $933.30
Per sample, 20-95C scan, 1C interval, 1C/min rate, 30s hold, 1-3 wavelengths, includes CD scan at 20C.

FY23 Example Project Fees

Example project details: Light Scattering analysis project for SEC-MALS of 2 proteins in one buffer system. Project setup includes column equilibration (12+ column volumes) into user-provided buffer and a BSA control experiment.  One additional replicate injection of each sample was requested.

line item Qty Internal price External Academic price
SEC-MALS setup  1 $450 $549.00
SEC-MALS data collection 2 2x $250 2x $305.00
SEC-MALS replicate injection 2 2x $110 2x $134.20
Total   $1,170 $1427.40

Nanobody Selection Fees

Please contact the CMI to start a conversation about nanobody selections.  Fees for Nanobody selections cover supplies and labor and are assessed for completed stages in the selection, regardless of final experimental outcome of the selection. 

Service

Harvard Internal

(33-digit)

External
Academic
Antigen Purification by SEC
Required if not completed by user
$650 $793.00
Yeast Surface Display Selection Campaign
Includes 2 rounds MACS, 2 rounds FACS and clonal isolation
delivery of any sequences or clones produced
$7980 $9735.60
Optional - Expression and Purification of Nanobody
1L scale, in e.coli pET26b, with 6-His tag
$4180 $5099.60
Total
includes selection campaign and nanobody expression
$12810 $15628.20