Data Collection Service Fees
Data Collection
- Limited Data Collection Services are offered.
- Service fees are based on labor and supplies costs, and will be charged for all completed services, regardless of experimental outcome.
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Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
- External Users will also be required to submit a PO and a signed CMI User Agreement.
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Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee.
- Some services include replicate measurements by default in the per-sample fee. For others, there is a reduced-price replicate measurement fee, if collected in the same dataset.
- Nanobody services not available to commercial users at this time.
- Current Harvard Life Lab commercial users are offered a 25% discount off the standard commercial rates.
Data Collection - FY24 Fees (Effective July 1, 2023)
Light Scattering Services
Dynamic Light Scattering (DLS) Data Collection Fees
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
DLS - 1 - Setup Fee, per plate | $275 | $330 | $660 |
Setup fee per plate, up to 48 samples, includes BSA control and 1 buffer control. In addition to per-sample fee. | |||
DLS - 2 - Sample Data Collection | $80 | $96 | $192 |
Per sample fee, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well, in addition to plate setup fee. | |||
DLS - 3 - Additional Replicate Sample | $45 | $54 | $108 |
Per additional replicate on the same plate |
Static Light Scattering (SEC-MALS) Data Collection Fees
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
SEC-MALS - 1 - Setup Fee, per buffer | $550 | $660 | $1,320 |
Per buffer, includes analytical column equilibration, BSA control, in addtion to per-sample fee. | |||
SEC-MALS - 2 - Sample Data Collection | $250 | $300 | $600 |
Per sample injection on analytical column, 0.5 ml/min. | |||
SEC-MALS - 3 - Replicate Injection of Sample | $125 | $150 | $300 |
Per replicate sample injection collected in the same sequence. | |||
SEC-MALS - dndc measurement | $540 | $648 | $1,296 |
Per user-provided reagent. |
Mass Photometry (MP) Data Collection Fees
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
Mass Photometry - 1 - Setup Fee | $370 | $444 | $888 |
Setup fee per calibration, per buffer, per FOV. | |||
Mass Photometry - 2 - Sample Data Collection | $190 | $228 | $456 |
Per sample fee, in triplicate. |
Differential Scanning Fluorimetry Services
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
Prometheus - 1 - Setup Fee using NanoDSF, per capillary set | $295 | $354 | $708 |
NanoDSF Setup fee per capillary set, up to 10 samples, includes lysozyme or BSA control. DSF on the Prometheus using protein fluorescence. In addition to per-sample fee. | |||
Prometheus - 2 - Sample Data Collection, in duplicate | $85 | $102 | $204 |
NanoDSF Per sample fee, measured in duplicate, 2C/min, in addition to setup fee. | |||
Prometheus - 2P - Sample Data Collection, in duplicate, HIGH SENSITIVITY CAPILLARIES | $110 | $132 | $264 |
NanoDSF Per sample fee, measured in duplicate, 2C/min, High Sensitivity Capillaries for low concentration | |||
Prometheus - 3 - Additional Replicate Sample | $25 | $30 | $60 |
NanoDSF per additional replicate collected in same cassette. | |||
Prometheus - 3P - Additional Replicate Sample, HIGH SENSITIVITY CAPILLARIES | $35 | $42 | $84 |
NanoDSF per additional replicate collected in same cassette, High Sensitivity Capillaries for low concentration. | |||
Prometheus - Buffer Optimization Screen | $635 | $762 | $1,524 |
NanoDSF 96 conditions NaCl vs pH, 1 measurement each, 2C/min | |||
QuantStudio - 1 - Setup Fee using Sypro Orange, per plate | $305 | $366 | $732 |
DSF Setup fee per plate, up to 30 samples, includes lysozyme control and 1 buffer control. DSF on the QuantStudio using SyproOrange protein stain. In addition to per-sample fee. | |||
QuantStudio - 2 - Sample Data Collection, in triplicate | $75 | $90 | $180 |
DSF Per sample fee, measured in triplicate, 2C/min, in addition to setup fee. | |||
QuantStudio - Buffer Optimization Screen | $485 | $582 | $1,164 |
DSF 96 conditions NaCl vs pH, 1 measurement each, 2C/min |
Circular Dichroism Services
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
CD - 1 - Scan Setup Fee | $220 | $264 | $528 |
Setup fee per dataset (up to 20 scans), includes 1 buffer control. Additional buffers should be listed as samples. | |||
CD - 2 - Sample Data Collection CD Scan | $140 | $168 | $336 |
Per sample, Far-UV 260-185 nm scan, 5-10 accumulations, in addition to setup fee. | |||
CD - 3 - Replicate Sample CD Scan | $65 | $78 | $156 |
Per Replicate sample collected in the same dataset (or alternate temperature scan of existing sample). | |||
CD - thermal denaturation | $515 | $618 | $1,236 |
Per sample, 20-95C scan, 1C interval, 1C/min rate, 30s hold, 1-3 wavelengths, includes CD scan at 20C. |
Chromatography Services
SEC Protein Purification Services - Preparative
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
SEC 10/300 - 1 - Setup Fee, per buffer | $460 | $552 | $1,104 |
per buffer, includes column equilibration and gel filtration standards (up to 12 injections on a 10/300) | |||
SEC 10/300 - 2 - Sample Purification | $200 | $240 | $480 |
SEC purification of one sample, per injection, up to 500 ul at 10 mg/ml. | |||
SEC 10/300 - 3 - Replicate purification | $110 | $132 | $264 |
Repeat injection of another sample in the set. | |||
SEC 10/300 - Optional - Special conditions - 1a -Setup | $205 | $246 | $492 |
Surcharge per setup, for low flow rate or other special condition. | |||
SEC 10/300 - Optional - Special conditions - 2a - Sample | $60 | $72 | $144 |
Surcharge per sample, for low flow rate or other special condition |
FSEC Data Collection Services - Analytical
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
FSEC - 1 - Setup Fee, per buffer | $270 | $324 | $648 |
Per buffer, includes 50 mm analytical column equilibration, BSA control, in addtion to per-sample fee. | |||
FSEC - 2 - Sample Data Collection | $80 | $96 | $192 |
Per sample injection on analytical column, 0.5 ml/min. | |||
FSEC - 3 - Replicate Injection of Sample | $50 | $60 | $120 |
Per replicate sample injection collected in the same sequence. |
Static Light Scattering (SEC-MALS) Data Collection Services - Analytical
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
SEC-MALS - 1 - Setup Fee, per buffer | $550 | $660 | $1,320 |
Per buffer, includes analytical column equilibration, BSA control, in addtion to per-sample fee. | |||
SEC-MALS - 2 - Sample Data Collection | $250 | $300 | $600 |
Per sample injection on analytical column, 0.5 ml/min. | |||
SEC-MALS - 3 - Replicate Injection of Sample | $125 | $150 | $300 |
Per replicate sample injection collected in the same sequence. | |||
SEC-MALS - dndc measurement | $540 | $648 | $1,296 |
Per user-provided reagent. |
General Services
Harvard base price |
Non-Harvard (base+F&A) | Commercial | |
Buffer Preparation 1L - PBS | $70 | $84 | $168 |
Up to 1 L PBS buffer, filtered and degassed | |||
Buffer Preparation 1L - User Custom Buffer | $165 | $198 | $396 |
Up to 1 L SEC buffer using reagents available at the CMI (phosphate, HEPES, Tris, NaCl reducing agent), filtered and degassed | |||
Buffer Exchange | $100 | $120 | $240 |
Protein buffer exchange into PBS or standard CD buffer (e.g. to 10 mM Phosphate, 150 mM NaF for CD) | |||
Custom Protein Services Hourly Labor (as available) | $135 | $162 | $324 |
Hourly Service Rate for custom protein services, in addition to consumables billed at cost. |
Example Project Fees
Example project details: Light Scattering analysis project for SEC-MALS of 2 proteins in one buffer system, with a replicate injection of each sample. Project setup includes column equilibration (12+ column volumes) into user-provided buffer and a BSA control experiment.
line item | Qty | Harvard Fee |
---|---|---|
SEC-MALS setup | 1 | $550 |
SEC-MALS data collection | 2 | 2x $250 |
SEC-MALS replicate injection | 2 | 2x $125 |
Total | $1,300 |
Nanobody Selection Services (ACADEMIC ONLY)
To learn more about nanobody selections at the CMI, visit our Nanobody Services page.
Fees for Nanobody selections cover supplies and labor and are assessed for completed stages in the selection, regardless of final experimental outcome of the selection.
Harvard base price | External Academic (base+F&A) | |
Antigen Purification by SEC | $660 | $792 |
Required, if not performed by user | ||
Basic Yeast Surface Display Nanobody Selection Campaign | $8,650 | $10,380 |
Includes 2 rounds MACS, 2 rounds FACS, clonal isolation, NGS of enriched library, delivery of any sequences or clones produced. | ||
Full Yeast Surface Display Nanobody Selection Campaign | $9,840 | $11,808 |
Includes Basic Yeast Surface Display Selection Campaign plus cloning into pET26b for periplasmic bacterial expression, for up to 20 isolated clones. | ||
Nanobody Expression and Purification | $4,410 | $5,292 |
Requires Full Yeast Surface Display Selection. Includes small scale periplasmic expression, purification and FSEC, then large scale express and purification of up to 3 Nb, if soluble. |
Data Collection Services
The CMI offers data collection services for assessing protein quality and sample biophysical properties. To start a conversation about a data collection services project, please contact the CMI or send a Sample Form to cmi@hms.harvard.edu.
Required Service Forms:
- Sample Form, for gathering sample details to aid in data collection. You may exclude proprietary information in your preliminary form (we'll let you know if essential information is missing).
- Academic Services Agreement, for EXTERNAL academic data collection service users
- OR Industry Services Agreement, for industry data collection service users