See also: Billing
Data Collection Service Fees
FY23 Fees - Data Collection (effective from July 1, 2022)
Data Collection Fees Summary
Data Collection Fees
- Limited Data Collection Services are offered. Service fees will be charged for all completed services, regardless of experimental outcome.
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Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
- External Users will also be required to submit a PO and a signed CMI User Agreement.
- Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee. Some services include one or more replicate measurements by default in the sample fee (e.g. DLS and DSF). For others, there may be reduced-price replicate measurement fee (e.g. SEC-MALS and CD) if collected in the same dataset.
Light Scattering Data Collection Fees
| Harvard base price | Non-Harvard (+F&A) | Commercial | Life Lab | |
| DLS - plate setup | $270.00 | $329.40 | $658.80 | $494.10 |
| Setup fee per plate, up to 48 samples, includes BSA control and 1 buffer control. In addition to per-sample fee. | ||||
| DLS - sample data collection - macromolecules | $75.00 | $91.50 | $183.00 | $137.25 |
| Per sample fee, in duplicate wells, 5-10 acquisitions/scan, 2 scans/well, in addition to plate setup fee. | ||||
| SEC-MALS -column setup | $450.00 | $549.00 | $1,098.00 | $823.50 |
| per buffer, includes analytical column equilibration, BSA control, in addtion to per-sample fee. | ||||
| SEC-MALS data collection | $250.00 | $305.00 | $610.00 | $457.50 |
| per sample, analytical column, 0.5 ml/min | ||||
| SEC-MALS replicate injection | $110.00 | $134.20 | $268.40 | $201.30 |
| replicate sample injection collected in the same sequence | ||||
| dn/dc measurement | $455.00 | $555.10 | $1,110.20 | $832.65 |
| per user-provided reagent | ||||
| SEC PBS Buffer preparation | $65.00 | $79.30 | $158.60 | $118.95 |
| up to 1 L PBS buffer, filtered and degassed | ||||
| SEC Custom Buffer preparation | $160.00 | $195.20 | $390.40 | $292.80 |
| up to 1 L custom buffer, made using reagents available at the CMI (HEPES, Phosphate, NaCl, reducing agents) |
DSF Data Collection Fees
| Harvard | Non-Harvard (+F&A) | Commercial | Life Lab | |
| base price | ||||
| DSF thermal shift analysis -plate setup | $ 275 | $ 335.50 | $ 671.00 | 503.25 |
| Setup fee per plate, up to 30 samples, includes lysozyme control and 1 buffer control. DSF on the QuantStudio using SyproOrange protein stain. In addition to per-sample fee. | ||||
| DSF sample data collection | $ 70 | $ 85.40 | $ 170.80 | 128.1 |
| Per sample fee, measured in triplicate, 2C/min, in addition to setup fee. | ||||
| DSF buffer optimization screen | $ 455 | $ 555.10 | $ 1,110.20 | 832.65 |
| 96 conditions NaCl vs pH, 1 measurement each, 2C/min | ||||
| nanoDSF thermal shift analysis -setup | $ 275 | $ 335.50 | $ 671.00 | $ 503.25 |
| Setup fee per capillary set, up to 10 samples, includes lysozyme or BSA control. DSF on the Prometheus using protein fluorescence. In addition to per-sample fee. | ||||
| nanoDSF sample data collection | $ 70 | $ 85.40 | $ 170.80 | $ 128.10 |
| Per sample fee, measured in duplicate, 2C/min, in addition to setup fee. | ||||
| Per sample fee, measured in duplicate, 2C/min, PREMIUM CAPILLARIES (High Sensitivity Capillaries for low concentration) | $ 110 | $ 134.20 | $ 268.40 | $ 201.30 |
| nanoDSF Buffer Optimization Screen | $ 595 | $ 725.90 | $ 1,451.80 | $ 1088.85 |
| 96 conditions NaCl vs pH, 1 measurement each, 2C/min |
CD Data Collection Fees
| Harvard base price | Non-Harvard (+F&A) | Commercial | Life Lab | |
| CD scan set up fee | $195 | $237.90 | $475.80 | $356.85 |
| Setup fee per dataset (up to 20 scans), includes 1 buffer control. Additional buffers should be listed as samples. | ||||
| CD scan for secondary structure | $135 | $164.70 | $329.40 | $247.05 |
| Per sample, Far-UV 260-185 nm scan, 5-10 accumulations, in addition to setup fee. | ||||
| CD replicate sample scan | $70 | $73.20 | $146.40 | $109.80 |
| Replicate sample collected in the same dataset (or alternate temperature scan of existing sample) | ||||
| CD thermal melt | $510 | $622.20 | $1,244.40 | $933.30 |
| Per sample, 20-95C scan, 1C interval, 1C/min rate, 30s hold, 1-3 wavelengths, includes CD scan at 20C. |
FY23 Example Project Fees
Example project details: Light Scattering analysis project for SEC-MALS of 2 proteins in one buffer system. Project setup includes column equilibration (12+ column volumes) into user-provided buffer and a BSA control experiment. One additional replicate injection of each sample was requested.
| line item | Qty | Internal price | External Academic price |
|---|---|---|---|
| SEC-MALS setup | 1 | $450 | $549.00 |
| SEC-MALS data collection | 2 | 2x $250 | 2x $305.00 |
| SEC-MALS replicate injection | 2 | 2x $110 | 2x $134.20 |
| Total | $1,170 | $1427.40 |
Nanobody Selection Fees
Please contact the CMI to start a conversation about nanobody selections. Fees for Nanobody selections cover supplies and labor and are assessed for completed stages in the selection, regardless of final experimental outcome of the selection.
| Service |
Harvard Internal (33-digit) |
External Academic |
|---|---|---|
|
Antigen Purification by SEC Required if not completed by user |
$650 | $793.00 |
|
Yeast Surface Display Selection Campaign Includes 2 rounds MACS, 2 rounds FACS and clonal isolation delivery of any sequences or clones produced |
$7980 | $9735.60 |
|
Optional - Expression and Purification of Nanobody 1L scale, in e.coli pET26b, with 6-His tag |
$4180 | $5099.60 |
|
Total includes selection campaign and nanobody expression |
$12810 | $15628.20 |