Size exclusion chromatography with multi-angle static light scattering (SEC-MALS) is used to measure masses of macromolecules. Size-exclusion chromatography (SEC) separates molecules based on hydrodynamic volume, but is dependent on similarity to a set of reference standards for accurate mass determination and fails for elongated or sticky proteins. MALS uses the intensity and the angular dependence of the scattered light to measure absolute molar mass and size of the molecules (root mean square radius, rg) in solution. Combining SEC and MALS in an SEC-MALS experiment allows for more accurate mass measurements that either method alone. An inline Quasi-Elastic Light Scattering (QELS), also called a Dynamic Light Scattering (DLS) detector, enables measurement of a hydrodynamic radius.
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