Light Scattering
Content tagged with Light Scattering
How do I interpret the reported uncertainty in my SEC-MALS experiment?
The reported uncertainties in ASTRA are statistical only, and do not include any of the many possible systematic errors that may be present. Examples are errors in dn/dc, the light scattering calibration constant, the RI and/or UV calibration constants...
Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)
Size Exclusion Chromatography with Multi-Angle Light Scattering is used to measure weight-averaged masses (Mw) of macromolecules in solution by measure the intensity of scattered light and concentration of a sample as it elutes from an SEC column. The...
Mass Photometry (MP)
The TwoMP from Refeyn for Mass Photometry is a single-particle static light scattering detector, used to measure molar mass in solution.
How pure does my protein sample need to be for analysis by DSF, CD or light scattering?
The more pure your sample is, the easier it is to interpret the results, as contaminating proteins may contribute to your overall signal in bulk measurements.
Differential Scanning Fluorimetry (DLS), Circular Dichroism (CD) and Dynamic Light Scattering...
How much protein is needed for molecular property analysis by DSF, CD, or light scattering?
The amount of protein needed will vary by method and sometimes by the mass of protein to be characterized. See CMI Service Protein Requirements for estimates and recommendations for the amount of protein needed for standard data collection.
Light Scattering Services
Services
Light Scattering
The Wyatt DynaPro Plate Reader III for Dynamic Light Scattering (DLS) measures hydrodynamic radius. The Wyatt/Agilent SEC-MALS system and Refeyn TwoMP Mass Photometry instrument measure Static Light Scattering (SLS) to determine molar mass.