DSF Services

DSF Service Overview

Differential Scanning Fluorimetry (DSF) with fluorescent dye or using intrinsic protein fluorescence

CMI DSF Instruments: Life Technologies Quant Studio 6/7 and NanoTemper Technologies Prometheus NT.Plex

  • Conventional DSF, protein thermal stability using hydrophobic dye Sypro Orange
  • NanoDSF, protein thermal stability using intrinsic protein fluorescence
  • Chemical denaturation
  • Buffer optimization by conventional DSF or NanoDSF

 

Data Collection Fees Summary

Data Collection Fees

  • Limited Data Collection Services are offered. Service fees will be charged for all completed services, regardless of experimental outcome. 
  • Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
    • External Users will also be required to submit a PO and a signed CMI User Agreement.
  • Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee. Some services include one or more replicate measurements by default in the sample fee (e.g. DLS and DSF). For others, there may be reduced-price replicate measurement fee (e.g. SEC-MALS and CD) if collected in the same dataset.

Data Collection Fee List

DSF Data Collection Fees

 

Harvard
base price

Non-Harvard (+F&A)

Commercial

Life Lab

DSF thermal shift analysis -plate setup

Setup fee per plate, up to 30 samples, includes lysozyme control and 1 buffer control.  DSF on the QuantStudio using SyproOrange protein stain. In addition to per-sample fee.

$250

$305.00

$610.00

$457.50

DSF sample data collection

Per sample fee, measured in triplicate, 2C/min, in addition to setup fee.

$60

$73.20

$146.40

$109.80

DSF buffer optimization screen

96 conditions NaCl vs pH, 1 measurement each, 2C/min

$455

$555.10

$1,110.20

$832.65

nanoDSF thermal shift analysis -setup

Setup fee per capillary set, up to 10 samples, includes lysozyme or BSA control. DSF on the Prometheus using protein fluorescence

$250

$305.00

$610.00

$457.50

nanoDSF sample data collection

Per sample fee, measured in duplicate, 2C/min, in addition to setup fee.

$60

$73.20

$146.40

$109.80

nanoDSF Buffer Optimization Screen  

96 conditions NaCl vs pH, 1 measurement each, 2C/min

$585

$713.70

$1,427.40

$1,070.55

  • Differential Scanning Fluorimetry (DSF) Assay

    also known as Protein Thermal Shift Assay

    DSF experiment performed on the QuantStudio 6/7 instrument using the hydrophobic dye, Sypro Orange from Life Technologies.

    DSF experiment

  • DSF Buffer Optimization Screen

    96 unique conditions using 8 salt concentrations from 0-1 M NaCl and 12 buffers from pH 4.5 to pH 9.5, including water.  Data collected on Prometheus NT.plex using protein intrinsic fluorescence at 350/330 nm.