DSF Services
DSF Service Overview
Differential Scanning Fluorimetry (DSF) with fluorescent dye or using intrinsic protein fluorescence
- Conventional DSF, protein thermal stability using hydrophobic dye Sypro Orange
- NanoDSF, protein thermal stability using intrinsic protein fluorescence
- Chemical denaturation
- Buffer optimization by conventional DSF or NanoDSF
Data Collection Fees Summary
Data Collection Fees
- Limited Data Collection Services are offered. Service fees will be charged for all completed services, regardless of experimental outcome.
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Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
- External Users will also be required to submit a PO and a signed CMI User Agreement.
- Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee. Some services include one or more replicate measurements by default in the sample fee (e.g. DLS and DSF). For others, there may be reduced-price replicate measurement fee (e.g. SEC-MALS and CD) if collected in the same dataset.
DSF Data Collection Fees
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Harvard |
Non-Harvard (+F&A) |
Commercial |
Life Lab |
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DSF thermal shift analysis -plate setup Setup fee per plate, up to 30 samples, includes lysozyme control and 1 buffer control. DSF on the QuantStudio using SyproOrange protein stain. In addition to per-sample fee. |
$250 |
$305.00 |
$610.00 |
$457.50 |
|
DSF sample data collection Per sample fee, measured in triplicate, 2C/min, in addition to setup fee. |
$60 |
$73.20 |
$146.40 |
$109.80 |
|
DSF buffer optimization screen 96 conditions NaCl vs pH, 1 measurement each, 2C/min |
$455 |
$555.10 |
$1,110.20 |
$832.65 |
|
nanoDSF thermal shift analysis -setup Setup fee per capillary set, up to 10 samples, includes lysozyme or BSA control. DSF on the Prometheus using protein fluorescence |
$250 |
$305.00 |
$610.00 |
$457.50 |
|
nanoDSF sample data collection Per sample fee, measured in duplicate, 2C/min, in addition to setup fee. |
$60 |
$73.20 |
$146.40 |
$109.80 |
|
nanoDSF Buffer Optimization Screen 96 conditions NaCl vs pH, 1 measurement each, 2C/min |
$585 |
$713.70 |
$1,427.40 |
$1,070.55 |
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Differential Scanning Fluorimetry (DSF) Assay
also known as Protein Thermal Shift Assay
DSF experiment performed on the QuantStudio 6/7 instrument using the hydrophobic dye, Sypro Orange from Life Technologies.
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DSF Buffer Optimization Screen
96 unique conditions using 8 salt concentrations from 0-1 M NaCl and 12 buffers from pH 4.5 to pH 9.5, including water. Data collected on Prometheus NT.plex using protein intrinsic fluorescence at 350/330 nm.
