DSF Services
DSF Service Overview
In addition to instrument training, the CMI is now offering basic protein Differential Scanning Fluorimetry services, including protein thermal shift analysis and buffer optimization.
Differential Scanning Fluorimetry (DSF) with fluorescent dye or using intrinsic protein fluorescence
Life Technologies Quant Studio 6/7
- Conventional DSF, protein thermal stability using hydrophobic dye Sypro Orange
- Buffer optimization
NanoTemper Technologies Prometheus NT.Plex
- NanoDSF, protein thermal stability using intrinsic protein fluorescence
- Chemical denaturation
- Buffer optimization
Data Collection Fees Summary
Data Collection
- Limited Data Collection Services are offered.
- Service fees are based on labor and supplies costs, and will be charged for all completed services, regardless of experimental outcome.
-
Before submitting samples for data collection, users must approve the estimated charges and be given a date and time for sample delivery.
- External Users will also be required to submit a PO and a signed CMI User Agreement.
-
Most CMI Data Collection Services include a setup fee plus a per-sample data collection fee.
- Some services include replicate measurements by default in the per-sample fee. For others, there is a reduced-price replicate measurement fee, if collected in the same dataset.
- Nanobody services not available to commercial users at this time.
- Current Harvard Life Lab commercial users are offered a 25% discount off the standard commercial rates.
Data Collection Services
The CMI offers data collection services for assessing protein quality and sample biophysical properties. To start a conversation about a data collection services project, please contact the CMI or send a Sample Form to cmi@hms.harvard.edu.
Required Service Forms:
- Sample Form, for gathering sample details to aid in data collection. You may exclude proprietary information in your preliminary form (we'll let you know if essential information is missing).
- Academic Services Agreement, for EXTERNAL academic data collection service users
- OR Industry Services Agreement, for industry data collection service users
Example DSF Buffer Optimization Screen
96 unique conditions using 8 salt concentrations from 0-1 M NaCl and 12 buffers from pH 4.5 to pH 9.5, including water. Data collected on Prometheus NT.plex using protein intrinsic fluorescence at 350/330 nm.
Example Protein Thermal Shift Assay
Differential Scanning Fluorimetry (DSF)
DSF experiment performed on the QuantStudio 6/7 instrument using the hydrophobic dye, Sypro Orange from Life Technologies.
