HOME / SERVICES / DATA COLLECTION SERVICES /

Molecular Properties Protein Requirements

Data Collection Material Requirements

Method Notes Buffer Notes

minimum volume
(μl)

recommended minimum
concentration 

Mass Photometry per sample, triplicate in storage buffer 50

Protein: ~200 nM
(diluted to 10-20 nM)
AAV: >1012 capsid/ml

SEC-MALS per injection in storage buffer 150 mg ~ 10/mass(KDa)*
DLS per sample, duplicates in storage buffer 150 mg ~ 10/mass(KDa)*
CD per sample in final buffer 300 ~ 0.1 mg/ml
NanoDSF per sample, duplicates in final buffer 50

~ 0.2 mg/ml Standard Capillaries
~ 0.05 mg/ml High Sensitivity Capillaries

NanoDSF buffer screen, 96 conditions in storage buffer 200 ~ 2 mg/ml
DSF, sypro per sample, triplicates in storage buffer 50 ~ 0.5 mg/ml
DSF, sypro buffer screen, 96 conditions in storage buffer 200 ~ 2 mg/ml
Nanobody Selection per antigen, standard selection   1 ml ~ 15 μM

 

* Light Scattering Intensity is proportional to mass. A higher concentration (in mg/ml) of protein is required for small proteins than for large proteins.  BSA, which is 67 KDa, and typically very pure, will typically give an excellent signal in MALS and DSL experiments at 2 mg/ml, and an acceptable signal at 0.2 mg/ml.  For a protein that is 1/2 the mass, plan use a concentration in the 0.4-4 mg/ml range.