Can I get the CMI Yeast Surface Display Nanobody Library to perform my own selections?
For Nanobody selections, the CMI currently uses a synthetic yeast display library, developed in the Kruse lab, a based on a consensus framework derived from llama antibody genes with variable complementary determining region loops (CDRs) designed from known nanobody structures.1
This library is available for non-profit research from Kerafast: EF0014-FP. For-profit organizations can license the library from the Harvard Office of Technology Development.
- McMahon, C. et al.Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nat Struct Mol Biol25,289–296 (2018).