I want to measure a Kd, how do I choose which technique is best?

The CMI has four primary technologies to quantify molecular interactions: Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI), Isothermal Titration Calorimetry (ITC) and MicroScale Thermophoresis (MST). Each technologies has its advantages and disadvantages. 

Some factors to consider when choosing a binding method include:

  • need for kinetic and/or equilibrium fits
  • the relative size of the interactants
  • the amount and concentration of samples
  • the ease of labeling and/or immobilizing interactants
  • the affinity of the interactants

There is no one best method for measuring molecular interactions. Request a consultation at the CMI to discuss which method might be best for your specific project.

See also: