Differential Scanning Fluorimetry
Content tagged with Differential Scanning Fluorimetry
How pure does my protein sample need to be for analysis by DSF, CD or light scattering?
The more pure your sample is, the easier it is to interpret the results, as contaminating proteins may contribute to your overall signal in bulk measurements.
Differential Scanning Fluorimetry (DLS), Circular Dichroism (CD) and Dynamic Light Scattering...
How much protein is needed for molecular property analysis by DSF, CD, or light scattering?
The amount of protein needed will vary by method and sometimes by the mass of protein to be characterized. See CMI Service Protein Requirements for estimates and recommendations for the amount of protein needed for standard data collection.
Are there fluorescent dyes than can be used for Differential Scanning Fluorimetry (DSF) of Membrane Proteins?
If your membrane protein has fluorescent residues (tryptophan and tyrosine), you should consider trying our Prometheus NT.plex for DSF using intrinsic protein fluorescence (NanoDSF).
However, if you wish to do conventional DSF (dye-based), please note that...
Services
Differential Scanning Fluorimetry (DSF)
The Prometheus NT.plex from NanoTemper and the QuantStudio from Life Technologies detect Differential Scanning Fluorimetry, used to measure protein thermal stability.