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Purification of α-synuclein from human brain reveals an instability of endogenous multimers as the protein approaches purity

Journal Article

Despite two decades of research, the structure-function relationships of endogenous, physiological forms of α-synuclein (αSyn) are not well understood. Most in vitro studies of this Parkinson's disease-related protein have focused on recombinant αSyn that...

Cell-Free Protein Synthesis

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The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip.

What is the best way to measure protein concentration?

FAQ

For purifed protein samples with aromatic residues, one of the most reliable ways to measure concentration is absorbance at 280, assuming you concentration is high enough for detection in the linear range of the spectrophometer.

measuring protein...

Chromatography Services

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The CMI offers protein purification services on the AKTA Pure Liquid Chromatography System with block fraction collection, using size-exclusion chromatography and ion-exchange chromatography. The CMI also offers analytical size-exclusion chromatography...

The CMI welcomes Ahmad Almawi

News

Ahmad Almawi, PhD, has joined the team at the CMI as a protein scientist. Ahmad trained as a structural biologist and has expertise in protein production and purification. He will be spearheading the nanobody production effort.

Fast Protein Liquid Chromatography (FPLC)

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The AKTA Pure from Cytiva is a preparative liquid chromatography system, used for protein purification by Size-Exclusion Chromatography and Ion Exchange.

Nuclera eProtein Discovery Installed

News

We are pleased to announce the installation of the Nuclera eProtein Discovery System at the CMI, allowing rapid protein prototyping using Cell-Free Protein Synthesis. The eProtein Discovery system automates the screen of up to 192 unique expression...

CMI Lunch Seminar. Nuclera eProtein Discovery System: Early Expression. More Publications.

Events

Learn more about the eProtein Discovery system from Nuclera , a novel platform for automated cell free protein synthesis, now available at the CMI! Seminar Agenda: How can eProtein Discovery™ help streamline your protein research? Description of the...

Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies.

Journal Article

Four nanobodies (VHH1-4) that target the yeast SUMO protein Smt3p were isolated and characterized. VHH1-4 bind to Smt3p and Smt3p-tagged proteins with high affinity (Kd: low nM). NMR analysis shows that the four nanobodies all bind near the C-terminus of...

N-Terminal Protein Labeling with N-Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein-Protein Interactions Using Labeled Protein

Journal Article

Protein labeling strategies have been explored for decades to study protein structure, function, and regulation. Fluorescent labeling of a protein enables the study of protein-protein interactions through biophysical methods such as microscale...

Seminar: Nuclera eProtein Discovery System

Events

Title: Accelerate Protein Production Speaker: Chava Angell, PhD, Nuclera Proteins are often the cornerstone and starting point of biological research. However, obtaining the proteins of interest can be difficult. Nuclera has developed the eProtein...

Identifying the Protein Interactions of the Cytosolic Iron-Sulfur Cluster Targeting Complex Essential for Its Assembly and Recognition of Apo-Targets

Journal Article

The cytosolic iron-sulfur cluster assembly (CIA) system assembles iron-sulfur (FeS) cluster cofactors and inserts them into >20 apoprotein targets residing in the cytosol and nucleus. Three CIA proteins, called Cia1, Cia2, and Met18 in yeast, form the...

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30 results for "Protein purification"

30 results for "Protein purification"

Purification of α-synuclein from human brain reveals an instability of endogenous multimers as the protein approaches purity

Cell-Free Protein Synthesis

What is the best way to measure protein concentration?

Chromatography Services

The CMI welcomes Ahmad Almawi

Fast Protein Liquid Chromatography (FPLC)

Nuclera eProtein Discovery Installed

CMI Lunch Seminar. Nuclera eProtein Discovery System: Early Expression. More Publications.

Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies.

N-Terminal Protein Labeling with N-Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein-Protein Interactions Using Labeled Protein

Seminar: Nuclera eProtein Discovery System

Identifying the Protein Interactions of the Cytosolic Iron-Sulfur Cluster Targeting Complex Essential for Its Assembly and Recognition of Apo-Targets