The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip.
The AKTA Pure from Cytiva is a preparative liquid chromatography system, used for protein purification by Size-Exclusion Chromatography and Ion Exchange.
For purifed protein samples with aromatic residues, one of the most reliable ways to measure concentration is absorbance at 280, assuming you concentration is high enough for detection in the linear range of the spectrophometer.
The amount of protein needed will vary by method and sometimes by the mass of protein to be characterized. See CMI Service Protein Requirements for estimates and recommendations for the amount of protein needed for standard data collection.
For a basic nanobody selection campaign, 1 ml antigen at 15 μM is needed. We require SEC-purified protein (and can purify your antigen by SEC if you cannot). See CMI Service Protein Requirements.