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Are there fluorescent dyes than can be used for Differential Scanning Fluorimetry (DSF) of Membrane Proteins?

FAQ

If your membrane protein has fluorescent residues (tryptophan and tyrosine), you should consider trying our Prometheus NT.plex for DSF using intrinsic protein fluorescence (NanoDSF).

However, if you wish to do conventional DSF (dye-based), please note that...

Covalently circularized nanodiscs for studying membrane proteins and viral entry

Journal Article

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for...

A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo

Journal Article

Movement of molecules across membranes in response to a stimulus is a key component of cellular programming. Here, we characterize and manipulate the response of a protein-based piston capable of puncturing membranes in a pH-dependent manner. Our protein...

Mechanism of membrane-curvature generation by ER-tubule shaping proteins

Journal Article

The endoplasmic reticulum (ER) network consists of tubules with high membrane curvature in cross-section, generated by the reticulons and REEPs. These proteins have two pairs of trans-membrane (TM) segments, followed by an amphipathic helix (APH), but how...

Cell-Free Protein Synthesis

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The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip. 

Effector-mediated membrane disruption controls cell death in CBASS antiphage defense

Journal Article

Cyclic oligonucleotide-based antiphage signaling systems (CBASS) are antiviral defense operons that protect bacteria from phage replication. Here, we discover a widespread class of CBASS transmembrane (TM) effector proteins that respond to antiviral...

Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion

Journal Article

The β-barrel assembly machine (Bam) complex folds and inserts integral membrane proteins into the outer membrane of Gram-negative bacteria. The two essential components of the complex, BamA and BamD, both interact with substrates, but how the two...

Highly Basic Clusters in the Herpes Simplex Virus 1 Nuclear Egress Complex Drive Membrane Budding by Inducing Lipid Ordering

Journal Article

During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This unusual process is mediated by the viral nuclear egress complex (NEC) that deforms the membrane around the capsid by...

Activity and Structural Dynamics of Human ABCA1 in a Lipid Membrane

Journal Article

The human ATP-binding cassette (ABC) transporter ABCA1 plays a critical role in lipid homeostasis as it extracts sterols and phospholipids from the plasma membrane for excretion to the extracellular apolipoprotein A-I and subsequent formation of high...

E46K-like α-synuclein mutants increase lipid interactions and disrupt membrane selectivity

Journal Article

Parkinson's disease (PD) is one of the most common neurodegenerative disorders, and both genetic and histopathological evidence have implicated the ubiquitous presynaptic protein α-synuclein (αSyn) in its pathogenesis. Recent work has investigated how...

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization

Journal Article

Mitochondrial sirtuins, SIRT3-5, are NAD-dependent deacylases and ADP-ribosyltransferases that are critical for stress responses. However, a comprehensive understanding of sirtuin targets, regulation of sirtuin activity, and the relationships between...

Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins

Journal Article

The biosynthesis of many eukaryotic proteins requires accurate targeting to and translocation across the endoplasmic reticulum membrane. Post-translational protein translocation in yeast requires both the Sec61 translocation channel, and a complex of four...