For a basic nanobody selection campaign, 1 ml antigen at 15 μM is needed. We require SEC-purified protein (and can purify your antigen by SEC if you cannot).
For Nanobody selections, the CMI currently uses a synthetic yeast display library, developed in the Kruse lab, a based on a consensus framework derived from llama antibody genes with variable complementary determining region loops (CDRs) designed from known nanobody structures.1
This library is available for non-profit research from...
Nanobody projects for external user conducted at the CMI are subject to an Academic User Agreement for Nanobody Services.
This user agreement incorporates both our standard core service agreement terms and an expanded MTA for the Kruse Lab Nanobody Library, including a description of the revenue sharing agreement required in the event of commercialization by the User or User Institution.
The User/User Institution is free to use the results of this service without restriction for...
