Center for Macromolecular Interactions
The Center for Macromolecular Interactions
The Center for Macromolecular Interactions (CMI) in the department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School has a mission to enhance basic research in the HMS community by providing scientific consultation, training and access to shared biophysical instruments for the characterization and analysis of macromolecules and their complexes. CMI resources include tools for characterizing molecular properties, for measuring molecular interactions and for protein production.
The facility currently offers training and access to instruments for Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR), Biolayer Interferometry (BLI), MicroScale Thermophoresis (MST), Differential Scanning Fluorimetry (DSF), Circular Dichroism (CD), Light Scattering, including size-exclusion chromatography with multi-angle light scattering (SEC-MALS), Dynamic Light Scattering (DLS), Mass Photometry (MP), and protein production, including Fast Protein Liquid Chromatography (FPLC) and Cell-free Protein Synthesis.
The CMI is primarily a shared instrument facility offering training and access on our technologies. The CMI also offers limited data collection services, including protein chromatography, select characterization of protein physical properties and yeast surface display nanobody selections.
CMI Events
Info Session on Refeyn MassFluidix HC device
Building C power outage
Demo of Sartorius Octet R8e
CMI News
CMI Rates for FY27 Posted
Tecan Spark Plate Reader Training and Access now available
FPLC Training and Access Now available
Newest Technologies at the CMI
Multimode Plate Reader
Spark Multimode Plate Reader from Tecan, configured with Absorbance mode, Luminescence mode, and enhanced Fusion Optics Fluorescence mode.
Cell-Free Protein Synthesis
The eProtein Discovery system from Nuclera is a platform for Cell-Free Protein Synthesis (CFPS), or in vitro transcription/translation, and enables rapid protein prototyping on a digital microfluidic chip.
Fast Protein Liquid Chromatography (FPLC)
The AKTA Pure from Cytiva is a preparative liquid chromatography system, used for protein purification by Size-Exclusion Chromatography and Ion Exchange.
Mass Photometry (MP)
The TwoMP from Refeyn for Mass Photometry is a single-particle static light scattering detector, used to measure molar mass in solution.
Recent CMI User Publications
- CMI users agree to acknowledge the support of "the Center for Macromolecular Interactions at Harvard Medical School" in publications arising from use of the facility. CMI RRID: SCR_018270.