For a basic nanobody selection campaign, 1 ml antigen at 15 μM is needed. We require SEC-purified protein (and can purify your antigen by SEC if you cannot).
For Nanobody selections, the CMI currently uses a synthetic yeast display library, developed in the Kruse lab, a based on a consensus framework derived from llama antibody genes with variable complementary determining region loops (CDRs) designed from known nanobody structures.1
This library is available for non-profit research from...
